Dio3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218428 Martinez ME, et al., Genomic imprinting variations in the mouse type 3 deiodinase gene between tissues and brain regions. Mol Endocrinol. 2014 Nov;28(11):1875-86
Assay type:	RT-PCR
MGI Accession ID:	MGI:7735066
Gene symbol:	Dio3
Gene name:	deiodinase, iodothyronine type III

Probe:	Dio3-pF, Dio3-pR mutated allele
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Dio3 m-/p+ neocortex	P7	TS28: neocortex	Present (a)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Dio3 m+/p- neocortex	P7	TS28: neocortex	Present (a)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Dio3 m-/p+ T/H/S	P7	TS28: corpus striatum TS28: hippocampus TS28: thalamus	Present (b)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Dio3 m+/p- T/H/S	P7	TS28: corpus striatum TS28: hippocampus TS28: thalamus	Present (b)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).

Notes:
(a) In the neocortex, the mutated Dio3 mRNA species reveals similar levels of expression in heterozygous mice, irrespective of the parental origin of the mutated allele.
(b) In thalamus/hippocampus/striatum, the pattern of allelic expression in Dio3 m-/p+ and Dio3 m+/p- mice still shows significant preferential expression from the paternally inherited allele.

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