Smo Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:353362 Walker MF, et al., GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade. PLoS Biol. 2024 Aug;22(8):e3002685
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7734742
Gene symbol:	Smo
Gene name:	smoothened, frizzled class receptor

Antibody:	anti-pSMO (7TM0239A)
Detection system:	Secondary antibody coupled to Alexa Fluor 568
Assay notes:	The antibody used in this assay recognizes the phosphorylated form of the protein.

Results

Specimen 7A Smo^bnb: embryonic day 9.5; Smo^bnb/Smo^bnb (more

)
Note: Ventral spinal cord. Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		7A pSMO Smo^bnb , 7A Merge Smo^bnb

Specimen 7A Smo^cbb: embryonic day 9.5; Smo^cbb/Smo^cbb (more

)
Note: Ventral spinal cord. Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7A pSMO Smo^cbb , 7A Merge Smo^cbb	The percentage of the neural tube stained in the hypomorph is less than in wild type.

7A pSMO Smo^WT ©

7A Smo^WT ©

Specimen 7A Smo^WT: embryonic day 9.5 (more

)
Note: Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7A Merge Smo^WT , 7A pSMO Smo^WT , 7A Smo^WT	The cilia in the ventral neural tube, where Shh levels are the highest, are prominently stained; those in the dorsal neural tube, where levels of Shh are low, are not.

Specimen 7A Ptch1^-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Ventral spinal cord. Triple labeled: green - Smo; magenta - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	7A Merge Ptch1^-/- , 7A pSMO Ptch1^-/-	The percentage of the neural tube stained in the mutant is greater than in wild type.

S9A Smo^WT ©

Specimen S9A Smo^WT: embryonic day 9.5 (more

)
Note: Dorsal spinal cord. Triple labeled: green - Smo; red - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		S9A Smo^WT , S9A pSMO Smo^WT , S9A Merge Smo^WT	The cilia in the ventral neural tube, where Shh levels are the highest, are prominently stained; those in the dorsal neural tube, where levels of Shh are low, are not.

Specimen S9A Smo^bnb: embryonic day 9.5; Smo^bnb/Smo^bnb (more

)
Note: Dorsal spinal cord. Triple labeled: green - Smo; red - Arl13b (assay MGI:7734749); blue - Hoechst.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		S9A Merge Smo^bnb , S9A pSMO Smo^bnb

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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