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Gene Expression Data
Assay Details
Assay
Reference: J:66266 Lewandoski M, et al., Fgf8 signalling from the AER is essential for normal limb development. Nat Genet. 2000 Dec;26(4):460-3
Assay type: RNA in situ
MGI Accession ID: MGI:7720858
Gene symbol: Fgf4
Gene name: fibroblast growth factor 4
Probe: Fgf4 probe
Probe preparation: RNA
Results
Specimen 4a normal: embryonic day 10.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present Regionally restricted 4a normal Expression domain does not extend to anterior or posterior limits of AER.
Specimen 4a mutant: embryonic day 10.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present Widespread 4a mutant Anterior and posterior expansion of expression domain. Expression throughout AER.
Specimen 4b normal: embryonic day 10.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 4b normal
Specimen 4b mutant: embryonic day 10.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 4b mutant
Specimen 4c normal: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Absent 4c normal
Specimen 4c mutant: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 4c mutant Expression persisted for at least 24 hours longer than in normal limb buds.
Specimen 4d normal: embryonic day 12.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: forelimb Absent 4d normal
Specimen 4d mutant: embryonic day 12.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: forelimb Present 4d mutant Expression persisted for at least 24 hours longer than in normal limb buds.
Specimen 4e normal: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Weak 4e normal
Specimen 4e mutant: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present 4e mutant Expression persisted for at least 24 hours longer than in normal limb buds.
Specimen 4f normal: embryonic day 12.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: hindlimb Trace 4f normal
Specimen 4f mutant: embryonic day 12.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: hindlimb Present 4f mutant Expression persisted for at least 24 hours longer than in normal limb buds.
Specimen 4g normal: embryonic day 13.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: hindlimb Absent 4g normal
Specimen 4g mutant: embryonic day 13.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: hindlimb Present 4g mutant Expression persisted for at least 24 hours longer than in normal limb buds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/28/2026
MGI 6.24 		The Jackson Laboratory