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Gene Expression Data
Assay Details
Assay
Reference: J:314574 Seo H, et al., Differential expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits during hippocampal development in the mouse. Mol Brain. 2015 Feb 27;8:13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7716871
Gene symbol: Hcn1
Gene name: hyperpolarization activated cyclic nucleotide gated potassium channel 1
Antibody: anti-HCN1 (APC-056)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1A,C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hippocampus Weak Regionally restricted 1A , 1C Expression in hippocampal region, more intense in intermediate zone than in venticular zone. Expression level lower than for Hcn2 and Hcn4.
Specimen 2A-1: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: fimbria hippocampus Present Regionally restricted 2A-1
TS27: dentate gyrus granule cell layer Present 2A-1
TS27: hippocampus pyramidal cell layer Present 2A-1
TS27: hippocampus stratum lucidum Present 2A-1
TS27: hippocampus stratum oriens Present 2A-1
TS27: hippocampus stratum radiatum Present 2A-1
TS27: hippocampus Present Regionally restricted 2A-1 Expression in cells migrating from ventricular zone, more prominent for Hcn1 and Hcn2 than for Hcn4.
Specimen 2A-2,C,D: postnatal day 7 (more )
Note: Double labeled: red - Hcn1; green - Gfap (assay MGI:7716875).
Structure Cell Type Level Pattern Image Note
TS28: dentate gyrus granule cell layer Present 2D , 2A-2 , 2C
TS28: hippocampus molecular layer Present 2D , 2A-2 , 2C
TS28: hippocampus pyramidal cell layer Present 2D , 2A-2 , 2C
TS28: hippocampus stratum lacunosum Present 2D , 2A-2 , 2C
TS28: hippocampus hippocampal neuron Present Regionally restricted 2D , 2A-2 , 2C Expression in neuronal somata.
TS28: hippocampus hippocampal astrocyte Absent 2D , 2C , 2A-2 No expression in astrocytes.
Specimen 3A,D: postnatal day 21 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: fimbria hippocampus Strong 3D , 3A Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus molecular layer Strong 3D , 3A Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus stratum lacunosum Strong 3D , 3A Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: CA1 pyramidal cell layer Present Regionally restricted 3D , 3A No expression in Pvalb-expressing cells in stratum pyramidale of CA1.
TS28: hippocampus stratum oriens Present Regionally restricted 3D , 3A Expression in most Pvalb-expressing cells in stratum oriens and stratum radiatum.
TS28: hippocampus stratum radiatum Present Regionally restricted 3D , 3A Expression in most Pvalb-expressing cells in stratum oriens and stratum radiatum.
Specimen 3E,H: postnatal day 21 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: fimbria hippocampus Strong 3H , 3E Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus molecular layer Strong 3H , 3E Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus stratum lacunosum Strong 3H , 3E Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: CA2 pyramidal cell layer Strong 3H , 3E Gradually stronger expression in stratum oriens, stratum pyramidale and stratum radiatum of CA2 and CA3 compared to CA1.
TS28: hippocampus stratum oriens Strong 3H , 3E Gradually stronger expression in stratum oriens, stratum pyramidale and stratum radiatum of CA2 and CA3 compared to CA1.
TS28: hippocampus stratum radiatum Strong 3H , 3E Gradually stronger expression in stratum oriens, stratum pyramidale and stratum radiatum of CA2 and CA3 compared to CA1.
TS28: hippocampus CA2 Present Regionally restricted 3E , 3H Expression in most Pvalb-expressing cells.
Specimen 3I,L: postnatal day 21 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: fimbria hippocampus Strong 3L , 3I Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus molecular layer Strong 3L , 3I Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus stratum lacunosum Strong 3L , 3I Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: CA3 pyramidal cell layer Strong 3L , 3I Gradually stronger expression in stratum oriens, stratum pyramidale and stratum radiatum of CA2 and CA3 compared to CA1.
TS28: hippocampus stratum oriens Strong 3L , 3I Gradually stronger expression in stratum oriens, stratum pyramidale and stratum radiatum of CA2 and CA3 compared to CA1.
TS28: hippocampus stratum radiatum Strong 3L , 3I Gradually stronger expression in stratum oriens, stratum pyramidale and stratum radiatum of CA2 and CA3 compared to CA1.
TS28: hippocampus CA3 Present Regionally restricted 3L , 3I Expression in most Pvalb-expressing cells.
Specimen 3M,P: postnatal day 21 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: dentate gyrus hilus Present Regionally restricted 3M , 3P Expression in most Pvalb-expressing cells.
Specimen 6A,D: postnatal day 21 (more )
Note: Double labeled: red - Hcn1; green - Dcx (assay MGI:7716877).
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Absent 6A , 6D
Specimen 7A,D: postnatal day 56 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: fimbria hippocampus Present 7D , 7A Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus CA1 Present 7D , 7A Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus molecular layer Present 7D , 7A Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus stratum lacunosum Present 7D , 7A Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: CA1 pyramidal cell layer Present Regionally restricted 7D , 7A No expression in Pvalb-expressing cells in stratum pyramidale of Ca1.
Specimen 7E,H: postnatal day 56 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present Regionally restricted 7H , 7E present / RR Expression in most Pvalb-expressing cells.
TS28: fimbria hippocampus Present 7H , 7E Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus molecular layer Present 7H , 7E Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus stratum lacunosum Present 7H , 7E Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: CA2 pyramidal cell layer Present 7E , 7H Gradually stronger expression in stratum pyramidale and stratum radiatum of CA2 compared to CA1.
TS28: hippocampus stratum radiatum Present 7E , 7H Gradually stronger expression in stratum pyramidale and stratum radiatum of CA2 compared to CA1.
Specimen 7I,L: postnatal day 56 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present Regionally restricted 7L , 7I Expression in most Pvalb-expressing cells.
TS28: fimbria hippocampus Present 7I , 7L Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus molecular layer Present 7I , 7L Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: hippocampus stratum lacunosum Present 7I , 7L Strongest expression in stratum lacunosum-moleculare and alveus.
TS28: CA2 pyramidal cell layer Present 7I , 7L Gradually stronger expression in stratum pyramidale and stratum radiatum of CA2 compared to CA1.
TS28: hippocampus stratum radiatum Present 7I , 7L Gradually stronger expression in stratum pyramidale and stratum radiatum of CA2 compared to CA1.
Specimen 7M,P: postnatal day 56 (more )
Note: Double labeled: red - Hcn1; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: dentate gyrus hilus Present Regionally restricted 7P , 7M Expression in most Pvalb-expressing cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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