Gene Expression Data
Assay Details
Assay
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Reference:
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J:154889
Deschamps C, et al., Expression of ephrinA5 during development and potential involvement in the guidance of the mesostriatal pathway. Exp Neurol. 2009 Oct;219(2):466-80
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7714978
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Gene symbol:
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Efna5
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Gene name:
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ephrin A5
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Probe:
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ephrinA5 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4a:
embryonic day 12.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Strong |
Regionally restricted |
4a
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Mainly expressed in the ventral mesencephalon dorsally to the TH+ somas.
|
TS20: brainstem nucleus |
Strong |
Regionally restricted |
4a
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Expressed in the red nucleus.
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Specimen
4b:
embryonic day 12.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Specimen
4c:
embryonic day 12.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: corpus striatum |
Present |
Graded |
4c
|
A ventro-dorsal expression gradient was seen.
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Specimen
4d:
embryonic day 14.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: red nucleus |
Strong |
|
4d inset
,
4d
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Mainly expressed in the ventral mesencephalon dorsally to the TH+ somas.
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Specimen
4e:
embryonic day 14.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4e:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Specimen
4f:
embryonic day 14.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4f:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: corpus striatum |
Present |
Graded |
4f
|
A dorso-ventral expression gradient was seen.
|
Specimen
4g:
embryonic day 16.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4g:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: red nucleus |
Strong |
|
4g
|
Mainly expressed in the ventral mesencephalon dorsally to the TH+ somas.
|
Specimen
4h:
embryonic day 16.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4h:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Specimen
4i:
embryonic day 16.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4i:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: corpus striatum |
Present |
Regionally restricted |
4i
|
Expression was more concentrated in the caudal part.
|
Specimen
4j:
embryonic day 18.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4j:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: red nucleus |
Strong |
|
4j
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Mainly expressed in the ventral mesencephalon dorsally to the TH+ somas.
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Specimen
4k:
embryonic day 18.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4k:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Specimen
4l:
embryonic day 18.5
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
4l:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: corpus striatum |
Present |
Regionally restricted |
4l
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Expression was more concentrated in the dorsal part.
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Specimen
5a:
postnatal day 0
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5a:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: red nucleus |
Present |
|
5a
|
Mainly expressed in the ventral mesencephalon dorsally to the TH+ somas.
|
Specimen
5b:
postnatal day 0
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 0
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Specimen
5c:
postnatal day 0
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5c:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Specimen
5d:
postnatal day 7
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5d:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 7
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: red nucleus |
Present |
|
5d
|
Expression was diminished at P7.
|
Specimen
5e:
postnatal day 7
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5e:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thalamus |
Present |
|
5e
|
Weaker expression at P7.
|
Specimen
5f:
postnatal day 7
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5f:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Specimen
5g:
postnatal month 4
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5g:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal month 4
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: red nucleus |
Absent |
|
5g
|
No expression in the adult.
|
Specimen
5h:
postnatal month 4
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5h:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal month 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thalamus |
Absent |
|
5h
|
Expression was not detected in the adult.
|
Specimen
5i:
postnatal month 4
(more )
Note: Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
Specimen
5i:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal month 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Efna5; brown - Th (assay MGI:7714977).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus striatum |
Absent |
|
5i
|
Expression was not detected in the adult.
|
|