Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75859
Wigle JT, et al., An essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype. EMBO J. 2002 Apr 2;21(7):1505-13
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7713281
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Gene symbol:
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Flt4
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Gene name:
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FMS-like tyrosine kinase 4
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Results
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Specimen
1C:
embryonic day 10.5; Prox1tm1Gco/Prox1 +
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: lymphatic system |
endothelial cell of lymphatic vessel |
Strong |
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1C
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High levels of expression in budding lymphatic endothelial cells.
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| TS17: cardinal vein |
blood vessel endothelial cell |
Present |
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1C
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Expression still detected in blood vascular endothelial cells.
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Specimen
2B:
embryonic day 12.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: red - Flt4; green - Prox1 reporter (assay MGI:7713272).
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Flt4; green - Prox1 reporter (assay MGI:7713272).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
endothelial cell of lymphatic vessel |
Strong |
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2B
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High levels of expression in lymphatic endothelial cells.
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| TS20: cardinal vein |
vein endothelial cell |
Weak |
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2B
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Lesser expression in blood vascular endothelial cells.
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Specimen
3B:
embryonic day 14.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: red - Flt4; green - Prox1 reporter (assay MGI:7713272).
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Flt4; green - Prox1 reporter (assay MGI:7713272).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
3B
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Expression overlapping with Prox1 reporter expression in lymphatic endothelial cells.
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Specimen
3B':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Flt4; green - Prox1 reporter (assay MGI:7713272).
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Specimen
6E:
embryonic day 11.5; Prox1tm1Gco/Prox1 +
(more )
Specimen
6E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Strong |
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6E
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Strong expression in lymphatic endothelial cells.
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| TS19: cardinal vein |
vein endothelial cell |
Weak |
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6E
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Weaker expression in blood vascular endothelial cells.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: cardinal vein |
vein endothelial cell |
Weak |
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6F
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Weak expression only in blood vascular endothelial cells.
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Analysis Tools