Gene Expression Data
Assay Details
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Assay
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Reference:
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J:268403
Zhou L, et al., Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development. FASEB J. 2017 Jan;31(1):132-147
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:7711927
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Gene symbol:
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Angptl4
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Gene name:
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angiopoietin-like 4
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Probe:
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Angptl4-pF, Angptl4-pR
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Probe preparation:
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labelled with SYBR green
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Assay notes:
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Quantitative RT-PCR. Relative expression was normalized to 18s rRNA. The normalized expression level in the WT was arbitrarily set as 1.
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Results
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Image: 7B
| P1
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TS27: heart
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Present
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2 µg; total RNA |
Not Specified |
| Not Specified |
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| P1
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TS27: heart
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Ambiguous
(a)
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2 µg; total RNA |
involves: 129S1/Sv |
Fen1tm5.1Bhsh/Fen1tm5.1Bhsh | Not Specified |
The moribund state of S187A was predicted based on their inactivity and failure to suck milk. Such newborns would die within a couple of hours.
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| P1
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TS27: heart
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Present
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2 µg; total RNA |
involves: 129S1/Sv |
Fen1tm5.1Bhsh/Fen1tm5.1Bhsh | Not Specified |
The live newborns were those that could actively suck milk.
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Notes:
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Analysis Tools
