Gene Expression Data
Assay Details
Assay
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Reference:
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J:269981
Marcucci F, et al., Distinct timing of neurogenesis of ipsilateral and contralateral retinal ganglion cells. J Comp Neurol. 2019 Jan 1;527(1):212-224
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7708043
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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clone 39.4D5
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
1a:
embryonic day 15.5
(more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E11.
Specimen
1a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E11.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
1a
,
1d
|
Expression was detected in postmitotic retinal ganglion cells (RGC's) in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. Few of the RGC's were co-labeled with EdU when injected at E11.
|
Specimen
1e:
embryonic day 15.5
(more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E12.
Specimen
1e:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E12.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
1e
,
1h
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. Few of the RGC's were co-labeled with EdU when injected at E12.
|
Specimen
1i:
embryonic day 15.5
(more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E13.
Specimen
1i:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E13.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
1i
,
1l
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. The number of RGC's co-labeled with EdU was higher when injected at E13 than when injected at previous ages.
|
Specimen
1m:
embryonic day 15.5
(more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E14.
Specimen
1m:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E14.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
1m
,
1p
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. The number of RGC's co-labeled with EdU was highest when injected at E14 than when injected at previous ages.
|
Specimen
not shown E14-16.5:
embryonic day 16.5
(more )
Note: EdU injected at E14.
Specimen
not shown E14-16.5:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
EdU injected at E14.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU when injected at E14 was similar between samples taken at E15.5 and E16.5.
|
Specimen
not shown E15-16.5:
embryonic day 16.5
(more )
Note: EdU injected at E15.
Specimen
not shown E15-16.5:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
EdU injected at E15.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU when injected at E15 was similar to when injected at E14.
|
Specimen
3a:
embryonic day 18.5
(more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E13.
Specimen
3a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E13.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3a
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. Some RGC's were co-labeled with EdU when injected at E13.
|
Specimen
3b:
embryonic day 18.5
(more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E14.
Specimen
3b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E14.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3b
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. The number of RGC's co-labeled with EdU was higher when injected at E14 than when injected at E13.
|
Specimen
3c:
embryonic day 18.5
(more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E15.
Specimen
3c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E15.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3c
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. RGC proliferation, as determined by co-label with EdU, during late development was substantial until E15 and decreased thereafter.
|
Specimen
3d:
embryonic day 18.5
(more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E16.
Specimen
3d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E16.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3d
|
Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. RGC proliferation, as determined by co-label with EdU, was decreased when injected at E16 than when injected at previous ages.
|
|