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Gene Expression Data
Assay Details
Assay
Reference: J:269981 Marcucci F, et al., Distinct timing of neurogenesis of ipsilateral and contralateral retinal ganglion cells. J Comp Neurol. 2019 Jan 1;527(1):212-224
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7708043
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: clone 39.4D5
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1a: embryonic day 15.5 (more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E11.
Structure Cell Type Level Pattern Image Note
TS23: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 1a , 1d Expression was detected in postmitotic retinal ganglion cells (RGC's) in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. Few of the RGC's were co-labeled with EdU when injected at E11.

Specimen 1e: embryonic day 15.5 (more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E12.
Structure Cell Type Level Pattern Image Note
TS23: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 1e , 1h Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. Few of the RGC's were co-labeled with EdU when injected at E12.

Specimen 1i: embryonic day 15.5 (more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E13.
Structure Cell Type Level Pattern Image Note
TS23: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 1i , 1l Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. The number of RGC's co-labeled with EdU was higher when injected at E13 than when injected at previous ages.

Specimen 1m: embryonic day 15.5 (more )
Note: Triple labeled: red - Isl1; blue - Zic2 (assay MGI:7708044); green - EdU. EdU injected at E14.
Structure Cell Type Level Pattern Image Note
TS23: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 1m , 1p Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Zic2 was seen in ipsilateral RGC's. The number of RGC's co-labeled with EdU was highest when injected at E14 than when injected at previous ages.

Specimen not shown E14-16.5: embryonic day 16.5 (more )
Note: EdU injected at E14.
Structure Cell Type Level Pattern Image Note
TS24: retina ganglion cell layer retinal ganglion cell Present Regionally restricted Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU when injected at E14 was similar between samples taken at E15.5 and E16.5.

Specimen not shown E15-16.5: embryonic day 16.5 (more )
Note: EdU injected at E15.
Structure Cell Type Level Pattern Image Note
TS24: retina ganglion cell layer retinal ganglion cell Present Regionally restricted Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. The number of RGC's co-labeled with EdU when injected at E15 was similar to when injected at E14.

Specimen 3a: embryonic day 18.5 (more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E13.
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 3a Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. Some RGC's were co-labeled with EdU when injected at E13.

Specimen 3b: embryonic day 18.5 (more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E14.
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 3b Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. The number of RGC's co-labeled with EdU was higher when injected at E14 than when injected at E13.

Specimen 3c: embryonic day 18.5 (more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E15.
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 3c Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. RGC proliferation, as determined by co-label with EdU, during late development was substantial until E15 and decreased thereafter.

Specimen 3d: embryonic day 18.5 (more )
Note: Triple labeled: red - Isl1; blue - Isl2 (assay MGI:7708045); green - EdU. EdU injected at E16.
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 3d Expression was detected in postmitotic retinal ganglion cells in the ventrotemporal retina. Co-label with Isl2 was seen in contralateral RGC's. RGC proliferation, as determined by co-label with EdU, was decreased when injected at E16 than when injected at previous ages.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory