Gene Expression Data
Assay Details
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Assay
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Reference:
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J:269981
Marcucci F, et al., Distinct timing of neurogenesis of ipsilateral and contralateral retinal ganglion cells. J Comp Neurol. 2019 Jan 1;527(1):212-224
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7708045
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Gene symbol:
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Isl2
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Gene name:
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insulin related protein 2 (islet 2)
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Antibody:
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Anti-Islet2
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
3a:
embryonic day 18.5
(more  )
Note: Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E13.
Specimen
3a:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E13.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3a
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Expression was detected in contralateral retinal ganglion cells (RGC's) in the ventrotemporal retina. Co-label with Isl1 was seen in postmitotic RGC's. Some contralateral RGC's were co-labeled with EdU when injected at E13.
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Specimen
3b:
embryonic day 18.5
(more )
Note: Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E14.
Specimen
3b:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E14.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3b
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Expression was detected in contralateral retinal ganglion cells (RGC's) in the ventrotemporal retina. Co-label with Isl1 was seen in postmitotic RGC's. More contralateral RGC's were co-labeled with EdU when injected at E14 than when injected at E13.
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Specimen
3c:
embryonic day 18.5
(more )
Note: Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E15.
Specimen
3c:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E15.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3c
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Expression was detected in contralateral retinal ganglion cells (RGC's) in the ventrotemporal retina. Co-label with Isl1 was seen in postmitotic RGC's. More contralateral RGC's were co-labeled with EdU when injected at E15 than when injected at E14.
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Specimen
3d:
embryonic day 18.5
(more )
Note: Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E16.
Specimen
3d:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
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Triple labeled: blue - Isl2; red - Isl1 (assay MGI:7708043); green - EdU. EdU injected at E16.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: retina ganglion cell layer |
retinal ganglion cell |
Present |
Regionally restricted |
3d
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Expression was detected in contralateral retinal ganglion cells (RGC's) in the ventrotemporal retina. Co-label with Isl1 was seen in postmitotic RGC's. The number of contralateral RGC's co-labeled with EdU when injected at E16 sharply decreased.
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