Antibody:	a-Synuclein (Ab-136) Antibody #21278
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Another anti-alpha-syn (4D6) gave similar results.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Strong	Regionally restricted	1C	Expressed in dentate gyrus.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		1E

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		2A
TS17: forebrain marginal layer	Present		2A
TS17: hindbrain marginal layer	Present		2A
TS17: midbrain marginal layer	Present		2A
TS17: nasal pit	Present		2A

Structure	Level	Pattern	Image	Note
TS20: forebrain marginal layer	Present		2B
TS20: olfactory epithelium	Present		2B
TS20: sensory ganglion	Present		2B
TS20: pons	Strong	Diffuse	2B
TS20: midbrain marginal layer	Present	Regionally restricted	2B	Expressed in the dorsal region.
TS20: midbrain	Present	Regionally restricted	2B	Expression in the ventral midbrain diffused.
TS20: cerebellum primordium	Very strong	Regionally restricted	2B	Expression was most prominent in the deep cerebellar nuclei.
TS20: medulla oblongata	Moderate		2B
TS20: spinal cord	Moderate		2B

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Strong		2C
TS22: cortical subplate	Strong		2C
TS22: midbrain	Present	Regionally restricted	2C	Expression in the dorsal midbrain diffused to the intermediate zone.
TS22: midbrain marginal layer	Present	Regionally restricted	2C	Expression in the dorsal region.

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present	Regionally restricted	2D	Expression diffused to the cortical region.
TS24: deep cerebellar nucleus	Weak		2D	Only slightly stained.

Structure	Level	Pattern	Image	Note
TS17: telencephalon marginal layer	Present	Regionally restricted	3A	Expressed in the marginal layer of the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3B	Expression became more prominent in the mitral layer.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb mitral cell layer	Present		3C

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb mitral cell layer	Present		3D

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	3E	No expression in the neocortex.

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Moderate		3F

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Strong		3G
TS22: cortical subplate	Strong		3G
TS22: cortical plate	Weak		3G	Lightly stained.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Strong		3H
TS24: cortical subplate	Strong		3H

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Strong	Regionally restricted	3I	Expressed in the primordial dentate gyrus and ventral hippocampal commissure. Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3J	Expressed in the nonnuclear compartments.
TS28: hippocampal commissure	Present	Regionally restricted	3J	Expressed in the nonnuclear compartments in the ventral region.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Strong		3K
TS27: main olfactory bulb mitral cell layer	Strong		3K
TS27: main olfactory bulb external plexiform layer	Strong		3K	The granular cell layer.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3L	Expression pattern diffused, with the most abundant in the subplate, and the immunoreactions became less abundant in the neuronal cell bodies and were more apparent in the neuropil. However, no immunoreactions were found in the ventricular zone.
TS27: cortical subplate	Strong		3L
TS27: cerebral cortex ventricular zone	Absent		3L

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Moderate	Regionally restricted	4A	Expressed in the upper rhombic lip (rostral roof of the hindbrain), where the cerebellar primordium is destined to be formed. Expressed in the nuclei.
TS17: midbrain	Present	Regionally restricted	4A	Expressed in the caudal midbrain. Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS20: midbrain marginal layer	Present	Regionally restricted	4B	Expressed in a layer of cells in the dorsal region. Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS22: midbrain lateral wall	Present	Scattered	4C	Positive cells of the dorsal midbrain migrated to the inner layers and were scattered in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS24: midbrain lateral wall	Present	Regionally restricted	4D	Expressed in the deep intermediate zone. Most of the expressions were found in the non-nuclear compartments.

Structure	Level	Pattern	Image	Note
TS20: brain nucleus	Strong	Regionally restricted	4E	Strongly expressed in the deep cerebellar nuclei neurons.

Structure	Level	Pattern	Image	Note
TS22: deep cerebellar nucleus	Very strong		4F	Expressed in the nuclei.
TS22: cerebellum gray matter	Weak	Regionally restricted	4F	The cortical regions were also lightly stained.

Structure	Level	Pattern	Image	Note
TS24: cerebellar cortex	Present		4G	Expression was condensed in the cortical regions.
TS24: deep cerebellar nucleus	Weak		4G

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Purkinje cell	Strong		4H	Predominantly expressed in the Purkinje cells, which are cell bodies sandwiched between two layers individually consisting of the upper and deep populations of presumptive granule neurons.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present	Regionally restricted	4I	Expressed in a subset of cells in the dorsal region. Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present	Regionally restricted	4J	Expressed in the marginal layer in the dorsal region. Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Strong	Regionally restricted	4K	Expressed in the ventral regions where substantia nigra and ventral tegmental area are derived from. Distributed in the cell bodies as well as in the processes. Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present		4L	Detected in the cytoplasm and the processes in a subset of cells. A dense positive nerve fiber network was formed. Expressed in the non-nuclear compartments.
TS28: ventral tegmental area	Present		4L	Detected in the cytoplasm and the processes in a subset of cells. A dense positive nerve fiber network was formed. Expressed in the non-nuclear compartments.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Moderate	Regionally restricted	4M	Expressed in the pons marginal zone, localized in the cell bodies as well as in the nerve fibers.

Structure	Level	Pattern	Image	Note
TS20: pons marginal layer	Strong		4N	Expressed in the nerve fibers.
TS20: pons	Strong	Regionally restricted	4N	Expressed in some cell bodies in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	4O	Expressed in the marginal zone

Structure	Level	Pattern	Image	Note
TS20: spinal cord marginal layer	Moderate		4P	Expressed in the neural fibers.
TS20: spinal cord	Moderate	Regionally restricted	4P	Expressed in a subset of cells of the intermediate zone.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Moderate		5A	Expression was distributed in the whole cell bodies and in the processes.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Very strong		5B	Expression was distributed in the whole cell bodies and in the processes. Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Very strong		5C	Most of the expression exhibited a non-nuclear location.

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Strong		5D	Expression was distributed in the whole cell bodies and in the processes.

Structure	Level	Pattern	Image	Note
TS22: glossopharyngeal ganglion	Moderate		5E	Expression was distributed in the whole cell bodies and in the processes.

Structure	Level	Pattern	Image	Note
TS24: brain nucleus	Very strong	Regionally restricted	5F	Expressed in the nerve fibers of anterior olfactory nucleus. Most of the expression exhibited a non-nuclear location.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Strong		5G	Expressed in the nuclei.
TS20: nasal cavity respiratory epithelium	Absent		5G

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Very strong		5H	Most of the expressions were found in the non-nuclear compartments.

Structure	Level	Pattern	Image	Note
TS28: retina nerve fiber layer	Present		5I

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present
TS15: future hindbrain	Present	Regionally restricted		Expressed in parts of those structures that later developed from it, including the cerebellum and the pons.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Weak	Regionally restricted		Expressed in the marginal zone of the medulla oblongata.
TS17: telencephalon	Weak	Regionally restricted		Expressed in the anterior olfactory nucleus. No expression in the olfactory ventricle, neocortex intermediate and ventricular zones.
TS17: olfactory epithelium	Weak
TS17: future spinal cord	Trace			Very low.
TS17: retina	Trace			Very low.

Structure	Level	Pattern	Image	Note
TS19: rhombic lip	Moderate	Regionally restricted		Expressed in the upper rhombic lip.
TS19: dorsal root ganglion	Moderate
TS19: facial ganglion	Moderate
TS19: olfactory epithelium	Moderate
TS19: trigeminal ganglion	Moderate
TS19: telencephalon	Moderate	Regionally restricted		Expressed in the anterior olfactory nucleus. Weak expression in the main olfactory bulb mitral cell layer. No expression in the olfactory ventricle, neocortex intermediate and ventricular zones.
TS19: future spinal cord	Weak
TS19: glossopharyngeal ganglion	Weak
TS19: retina	Weak
TS19: vagus ganglion	Weak
TS19: cranial ganglion	Weak	Regionally restricted		Expressed in vestibulo-cochlear ganglion.
TS19: hindbrain	Weak	Regionally restricted		Expressed in the medulla oblongata.
TS19: midbrain	Weak	Regionally restricted		Expressed in the substantia nigra. No expression in the periaqueductal gray area, inferior colliculus and superior colliculus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted		Expressed in the primordial hippocampus. Strong expression in main olfactory bulb mitral cell layer, very low expression in the granule and glomerular layers. No expression in the olfactory ventricle, caudate putamen, neocortex intermediate and ventricular zones. Weak expression in globus pallidus and accumbens nucleus, very low expression in the amygdaloid complex.
TS20: midbrain	Present	Regionally restricted		Expressed in the caudal midbrain. No expression in the periaqueductal gray area, inferior colliculus and superior colliculus.
TS20: hindbrain	Present	Regionally restricted		Expressed in the rostral hindbrain. Moderately expressed in the substantia nigra.
TS20: midbrain-hindbrain junction	Present	Regionally restricted		Expressed in the marginal layer in the dorsal region.
TS20: facial ganglion	Strong
TS20: trigeminal ganglion	Strong
TS20: vestibulo-cochlear ganglion	Strong
TS20: glossopharyngeal ganglion	Moderate
TS20: retina	Moderate
TS20: vagus ganglion	Moderate
TS20: brain nucleus	Very strong	Regionally restricted		Expressed in the anterior olfactory nucleus.
TS20: rhombic lip	Moderate	Regionally restricted		Expressed in the upper rhombic lip.
TS20: metencephalon alar plate	Trace	Regionally restricted		Expressed in the cerebellum external germinative cell layer.
TS20: cerebral cortex intermediate zone	Absent
TS20: cerebral cortex ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS22: pons	Strong	Regionally restricted		Expressed in some cell bodies in the intermediate zone.
TS22: spinal cord lateral wall	Strong	Regionally restricted		Expressed in some cell bodies in the intermediate zone.
TS22: pons marginal layer	Strong			More extensive expression in the nerve fibers.
TS22: spinal cord marginal layer	Strong			More extensive expression in the nerve fibers.
TS22: brain nucleus	Very strong	Regionally restricted		Expressed in the anterior olfactory nucleus.
TS22: facial ganglion	Strong
TS22: medulla oblongata	Strong
TS22: retina	Strong
TS22: vestibulo-cochlear ganglion	Strong
TS22: globus pallidus	Moderate
TS22: vagus ganglion	Moderate
TS22: olfactory epithelium	Very strong
TS22: cerebellum gray matter	Weak	Regionally restricted		Expressed in the cerebellum external germinative cell layer.
TS22: midbrain	Present	Regionally restricted		No expression in the periaqueductal gray area, inferior colliculus and superior colliculus.
TS22: main olfactory bulb mitral cell layer	Very strong
TS22: caudate-putamen	Absent
TS22: cerebral cortex ventricular layer	Absent
TS22: main olfactory bulb ventricular layer	Absent
TS22: main olfactory bulb layer	Trace	Regionally restricted		Very low expression in the granule and glomerular layers.
TS22: cerebral cortex intermediate zone	Trace
TS22: telencephalon	Weak	Regionally restricted		Weak expression in accumbens nucleus and amygdaloid complex.

Structure	Level	Pattern	Image	Note
TS24: facial ganglion	Strong
TS24: globus pallidus	Strong
TS24: hippocampus	Strong
TS24: medulla oblongata	Strong
TS24: retina	Strong
TS24: spinal cord	Strong
TS24: trigeminal ganglion	Strong
TS24: vagus ganglion	Strong
TS24: vestibulo-cochlear ganglion	Strong
TS24: hippocampal commissure	Strong	Regionally restricted		Expressed in the ventral region.
TS24: pons marginal layer	Strong			Expressed in the nerve fibers.
TS24: pons	Strong	Regionally restricted		Expressed in some cell bodies in the intermediate zone.
TS24: cerebellum external granule cell layer	Moderate
TS24: glossopharyngeal ganglion	Moderate
TS24: midbrain	Very strong	Regionally restricted		Expressed in the substantia nigra. Weak expression in inferior colliculus and superior colliculus. Very low expression in the periaqueductal gray area.
TS24: caudate-putamen	Absent
TS24: cerebral cortex ventricular layer	Absent
TS24: main olfactory bulb ventricular layer	Absent
TS24: dentate gyrus primordium	Very strong
TS24: main olfactory bulb mitral cell layer	Very strong
TS24: cerebral cortex intermediate zone	Weak
TS24: cortical plate	Weak
TS24: main olfactory bulb prospective glomerular layer	Weak
TS24: main olfactory bulb prospective granule cell layer	Weak
TS24: brain nucleus	Moderate	Regionally restricted		Expressed in accumbens nucleus, low expression in the amygdaloid complex.

Structure	Level	Pattern	Image	Note
TS26: cerebellum external granule cell layer	Strong
TS26: cerebral cortex marginal zone	Strong
TS26: facial ganglion	Strong
TS26: globus pallidus	Strong
TS26: main olfactory bulb glomerular layer	Strong
TS26: main olfactory bulb prospective granule cell layer	Strong
TS26: medulla oblongata	Strong
TS26: pons	Strong
TS26: retina	Strong
TS26: spinal cord	Strong
TS26: trigeminal ganglion	Strong
TS26: vagus ganglion	Strong
TS26: vestibulo-cochlear ganglion	Strong
TS26: main olfactory bulb external plexiform layer	Strong			The granular cell layer.
TS26: cerebral cortex	Present	Regionally restricted		Expression pattern diffused, with the most abundant in the subplate, and the immunoreactions became less abundant in the neuronal cell bodies and were more apparent in the neuropil. However, no immunoreactions were found in the ventricular zone.
TS26: cortical subplate	Strong
TS26: caudate-putamen	Absent
TS26: cerebral cortex ventricular zone	Absent
TS26: hippocampus	Present	Widespread		Expression became extensive.
TS26: hippocampal commissure	Strong	Regionally restricted		Expression became extensive in the ventral region.
TS26: midbrain	Present	Regionally restricted		Expression diffused throughout the neuroepithelium of the dorsal midbrain. Weak expression in the periaqueductal gray area, inferior colliculus and superior colliculus.
TS26: deep cerebellar nucleus	Weak
TS26: cerebellar cortex	Present			Expression was condensed in the cortical regions.
TS26: dentate gyrus	Very strong
TS26: dorsal root ganglion	Very strong
TS26: olfactory epithelium	Very strong
TS26: substantia nigra	Very strong
TS26: accumbens nucleus	Moderate
TS26: cerebral cortex intermediate zone	Moderate
TS26: cortical plate	Moderate
TS26: glossopharyngeal ganglion	Moderate
TS26: brain nucleus	Very strong	Regionally restricted		Expressed in the anterior olfactory nucleus. Low expression in the amygdaloid complex.
TS26: main olfactory bulb mitral cell layer	Very strong
TS26: main olfactory bulb ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present	Widespread		Expression became extensive.
TS27: hippocampal commissure	Strong	Regionally restricted		Expression became extensive in the ventral region.
TS27: midbrain	Present	Regionally restricted		Expression diffused throughout the neuroepithelium of the dorsal midbrain. Moderate expression in the periaqueductal gray area, inferior colliculus and superior colliculus.
TS27: dentate gyrus	Very strong
TS27: main olfactory bulb mitral cell layer	Very strong
TS27: olfactory epithelium	Very strong
TS27: substantia nigra	Very strong
TS27: cerebellum external granule cell layer	Strong
TS27: cerebral cortex marginal zone	Strong
TS27: globus pallidus	Strong
TS27: main olfactory bulb glomerular layer	Strong
TS27: main olfactory bulb granule cell layer	Strong
TS27: medulla oblongata	Strong
TS27: pons	Strong
TS27: retina	Strong
TS27: spinal cord	Strong
TS27: amygdaloid nucleus	Weak
TS27: deep cerebellar nucleus	Weak
TS27: caudate-putamen	Absent
TS27: main olfactory bulb ventricular layer	Absent
TS27: accumbens nucleus	Moderate
TS27: cerebral cortex intermediate zone	Moderate
TS27: cortical plate	Moderate