Antibody:	ARF1 Polyclonal antibody (10790-1-AP)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The antibody used recognized three family members; all family members have been annotated.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Diffuse	4a	Diffusely distributed throughout the cerebral cortex.
TS28: cerebral hemisphere white matter	Trace		4a , 4e	Barely detected.
TS28: cortical layer I	Present	Diffuse	4b , 4a
TS28: cortical layer II	Present	Diffuse	4c , 4a	Expression was not localized in the nucleus of cortical neurons, the borderline between positive cytoplasmic and -negative nuclear regions was vague.
TS28: cortical layer III	Present	Diffuse	4c , 4a	Expression was not localized in the nucleus of cortical neurons, the borderline between positive cytoplasmic and -negative nuclear regions was vague.
TS28: cortical layer V	Present	Diffuse	4a , 4d	Expression was not localized in the nucleus of cortical neurons, the borderline between positive cytoplasmic and -negative nuclear regions was vague.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Moderate	Regionally restricted	4g , 4h , 4i	Expression was barely observed in axon bundles, moderate immunoreactivity was detected in its axon-poor region where inhibitory medium spiny neurons are mainly present.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus	pyramidal neuron	Present	Widespread	4j	Enriched in the dendrites of pyramidal neurons.
TS28: hippocampus CA1	pyramidal neuron	Present	Widespread	4j	Enriched in the dendrites of pyramidal neurons.
TS28: hippocampus CA2	pyramidal neuron	Present	Widespread	4j	Enriched in the dendrites of pyramidal neurons.
TS28: hippocampus CA3	pyramidal neuron	Present	Widespread	4j	Enriched in the dendrites of pyramidal neurons.
TS28: hippocampus stratum oriens	pyramidal neuron	Present		4k , 4j	Where basal dendrites of pyramidal neurons are found. Barely present in axons, neuronal cell bodies and nuclei.
TS28: hippocampus stratum radiatum	pyramidal neuron	Present		4l , 4j	Where apical dendrites of pyramidal neurons are found.
TS28: dentate gyrus		Present	Regionally restricted	4j , 4m	Barely present in axons, neuronal cell bodies and nuclei.
TS28: hippocampal formation		Present	Regionally restricted	4j , 4n	Barely present in axons, neuronal cell bodies and nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Moderate		4p , 4o	Expressed in the molecular layer containing axons of granule cells and dendrites of Purkinje cells.
TS28: cerebellum Purkinje cell layer	Weak	Regionally restricted	4q , 4o	Barely detected in Purkinje cells.
TS28: cerebellum granule cell layer	Weak		4r , 4o	Barely detected.
TS28: cerebellum white matter	Trace		4s , 4o	Barely detected.