Myf5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:87223 Buttitta L, et al., Interplays of Gli2 and Gli3 and their requirement in mediating Shh-dependent sclerotome induction. Development. 2003 Dec;130(25):6233-43
Assay type:	RNA in situ
MGI Accession ID:	MGI:7703218
Gene symbol:	Myf5
Gene name:	myogenic factor 5

Probe:	Myf5 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

3E ©

Specimen 3E: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		3E
TS15: dermomyotome	Present	Regionally restricted	3E	Expression is in the dorsomedial lip.

3F ©

Specimen 3F: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Diffuse	3F	Expression in this mutant expanded laterally and the dorsomedial-specific expression was absent.

3O ©

Specimen 3O: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		3O

3P ©

Specimen 3P: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS15: somite	Present		3P	Expression is throughout the mesenchymal cells within the epithelial spheres.

3Q ©

Specimen 3Q: embryonic day 9.5; Shh^tm1.1Jhdk/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS15: myotome	Present	Non-Uniform	3Q	In this mutant the dorsomedial expression is reduced.

3R ©

Specimen 3R: embryonic day 9.5; Gli3^Xt-J/Gli3^Xt-J, Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		3R	Expression is less organized than in the wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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