Probe:	ngn1 genomic3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Present		1C +/+
TS16: trigeminal ganglion	Present		1C +/+

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Absent		1C -/-
TS16: trigeminal ganglion	Absent		1C -/-

Structure	Level	Pattern	Image	Note
TS13: trigeminal placode	Strong		2A	Strong expression seen first in trigeminal placode at E8.5.
TS13: future midbrain	Present		2A	Reciprocal pattern of Neurog1 and Neurog2 expression in midbrain and diencephalon.
TS13: future prosencephalon	Absent		2A	Reciprocal pattern of Neurog1 and Neurog2 expression in midbrain and diencephalon.

Structure	Level	Pattern	Image	Note
TS14: otic placode	Strong		2C	Strong expression in otic placode at E9.0.
TS14: trigeminal placode	Present		2C	In trigeminal placode, neuronal precursor cells delaminate, migrate and aggregate together to form the trigeminal ganglion.
TS14: geniculate placode	Weak		2C	Weak expression in geniculate (VII) and petrosal (IX) placodes.
TS14: petrosal placode	Weak		2C	Weak expression in geniculate (VII) and petrosal (IX) placodes.
TS14: epibranchial placode	Weak	Regionally restricted	2C	Weak expression in nodose (X) placode.

Structure	Level	Pattern	Image	Note
TS15: otic pit	Present	Regionally restricted	2E	Neuronal precursor cells in vestibulocochlear ganglia delaminate from otic placode.
TS15: trigeminal placode	Present		2E
TS15: geniculate placode	Weak		2E	Weak expression in geniculate (VII), petrosal (IX) and nodose (X) placodes.
TS15: nodose placode	Weak		2E	Weak expression in geniculate (VII), petrosal (IX) and nodose (X) placodes.
TS15: petrosal placode	Weak		2E	Weak expression in geniculate (VII), petrosal (IX) and nodose (X) placodes.

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present	Regionally restricted	2G	Expression in crest-derived accessory, superior, jugular (superior vagus, X) and trigeminal (V) ganglia.
TS17: trigeminal ganglion	Present		2G

Structure	Level	Pattern	Image	Note
TS12: trigeminal placode	Absent		4A	Stage just before expression is detected in trigeminal placode.

Structure	Level	Pattern	Image	Note
TS13: trigeminal placode	Strong		4B	Strong expression in trigeminal placode.

Structure	Level	Pattern	Image	Note
TS13: trigeminal placode	Present		6A

Structure	Level	Pattern	Image	Note
TS13: trigeminal placode	Present		8A	Expression in trigeminal region.

Structure	Level	Pattern	Image	Note
TS13: trigeminal placode	Present	Regionally restricted	8B	Density of expressing cells in trigeminal region appears not to be increased compared to wild type.
TS13: future spinal cord	Present		8B	Increase in expression in spinal cord.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		8C	Expression in midbrain and trigeminal regions.
TS13: trigeminal placode	Present		8C	Expression in midbrain and trigeminal regions.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Strong	Regionally restricted	8D	Density of expressing cells in midbrain region greatly increased compared to wild type.
TS13: trigeminal placode	Present	Regionally restricted	8D	Very slight, if any, increase in density or number of cells expressing high levels may be apparent in trigeminal region.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		8E	Expression in midbrain and trigeminal regions.
TS13: trigeminal placode	Present		8E	Expression in midbrain and trigeminal regions.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Strong	Regionally restricted	8F	Density of expressing cells in midbrain region greatly increased compared to wild type.
TS13: trigeminal placode	Present	Regionally restricted	8F	Very slight, if any, increase in density or number of cells expressing high levels may be apparent in trigeminal region.