Gene Expression Data
Assay Details
Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664561
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Gene symbol:
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Pou6f2
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Gene name:
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POU domain, class 6, transcription factor 2
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Probe:
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Pou6f2_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
5k:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5k:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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Specimen
7bb:
embryonic day 15.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
7bb:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 15.5
|
Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mesomere 2 |
Present |
Regionally restricted |
7bb
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Expression in the superficial preisthmic mantle stratum was detected in a curved shape above the isthmic superficial elements with a ventral head-like thickening (oriented topographically rostral) and a longer dorsal tail portion (oriented topographically caudal).
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Specimen
8aa:
embryonic day 15.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
8aa:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Male
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Type:
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section
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Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Sample was postfixed in 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mesomere 2 |
Present |
Regionally restricted |
8aa
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Selective labeling was observed in the basal or tegmental preisthmus.
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