Gene Expression Data
Assay Details
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Assay
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Reference:
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J:314568
Haara O, et al., Ectodysplasin regulates activator-inhibitor balance in murine tooth development through Fgf20 signaling. Development. 2012 Sep;139(17):3189-99
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7663903
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
7A:
embryonic day 13.5
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lower jaw 1st molar |
Present |
Regionally restricted |
7A
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A small dot of expression was detected in the anterior end of molar 1 in one third of the wild type E13.5 jaws.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Fgf20tm1.1Dor/Fgf20+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lower jaw 1st molar |
Present |
Regionally restricted |
7B
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A small dot of expression was detected in the anterior end of molar 1 in 50 % of the Fgf20<+/bgal> E13.5 jaws.
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Specimen
7C:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Fgf20tm1.1Dor/Fgf20tm1.1Dor
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lower jaw 1st molar |
Present |
Regionally restricted |
7C
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A small dot of expression was detected in the anterior end of molar 1 in all Fgf20<bgal/bgal> E13.5 jaws.
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Specimen
7G:
embryonic day 14.5
(more )
Specimen
7G:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw 1st molar |
Present |
Regionally restricted |
7G
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At E14.5, the expression in the small focus of anterior end molar 1 was lost in wild type mice. Other regions of expression continued.
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Specimen
7H:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Fgf20tm1.1Dor/Fgf20+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw 1st molar |
Present |
Regionally restricted |
7H
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The small dot of expression was detected in the anterior end of molar 1 in >80% of the Fgf20<+/bgal> E14.5 jaws.
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Specimen
7I:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Fgf20tm1.1Dor/Fgf20tm1.1Dor
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw 1st molar |
Present |
Regionally restricted |
7I
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The small dot of expression was detected in the anterior end of molar 1 in all of the Fgf20<bgal/bgal> E14.5 jaws.
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Analysis Tools
