Gene Expression Data
Assay Details
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Assay
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Reference:
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J:150882
Ichikawa H, et al., Brn-3a deficiency transiently increases expression of calbindin D-28 k and calretinin in the trigeminal ganglion during embryonic development. Cell Mol Neurobiol. 2009 Jul;29(5):691-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7662883
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Gene symbol:
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Calca
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Gene name:
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calcitonin/calcitonin-related polypeptide, alpha
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Antibody:
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anti-CGRP
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3D:
embryonic day 12.5
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3E:
embryonic day 14.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: trigeminal ganglion |
Weak |
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3E
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Decreased number of expressing cells compared to wild type at E12.5.
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Specimen
3F:
embryonic day 16.5
(more )
Specimen
3F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: trigeminal ganglion |
Present |
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3F
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Increased number of expressing cells compared to wild type at E14.5.
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Pou4f1tm1Nat/Pou4f1tm1Nat
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3K:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Pou4f1tm1Nat/Pou4f1tm1Nat
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: trigeminal ganglion |
Weak |
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3K
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Number of expressing cells not affected compared to wild type.
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Specimen
3L:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Pou4f1tm1Nat/Pou4f1tm1Nat
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: trigeminal ganglion |
Present |
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3L
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Number of expressing cells not affected compared to wild type.
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Analysis Tools