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Gene Expression Data
Assay Details
Assay
Reference: J:45668 Bergemann AD, et al., Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 1998 Jan 29;16(4):471-80
Assay type: RNA in situ
MGI Accession ID: MGI:7662604
Gene symbol: Efnb3
Gene name: ephrin B3
Probe: Efnb3 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Two Efnb3 probes were described, but the one used in this assay was not detailed. Probe name MGI:7662589 may have been used in this assay.
Results
Specimen 5A: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: future hindbrain floor plate Strong 5A Strongest expression in floor plate of midbrain and hindbrain.
TS13: future midbrain floor plate Strong 5A Strongest expression in floor plate of midbrain and hindbrain.
TS13: rhombomere 2 Weak 5A Weaker expression in dorsal hindbrain, in rhombomeres 2 and 4.
TS13: rhombomere 4 Weak 5A Weaker expression in dorsal hindbrain, in rhombomeres 2 and 4.
TS13: rhombomere Weak Regionally restricted 5A Weak expression in in rhombomeres 6.
Specimen 5B: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future hindbrain floor plate Strong 5B Expression most intense in floor plate of midbrain and hindbrain.
TS15: future midbrain floor plate Strong 5B Expression most intense in floor plate of midbrain and hindbrain.
Specimen 5C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindbrain floor plate Present 5C
Specimen 5D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: hindbrain floor plate Present 5D
Specimen 5E: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain floor plate Present 5E
TS20: spinal cord floor plate Present 5E
Specimen 5F: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: hindbrain floor plate Present 5F
TS23: hindbrain Present Regionally restricted 5F Expression in dorsal midline of hindbrain and spinal cord.
TS23: spinal cord Present Regionally restricted 5F Expression in dorsal midline of hindbrain and spinal cord.
Specimen 5G,H: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: hindbrain floor plate Present 5G , 5H
TS24: hindbrain Present Regionally restricted 5H , 5G Expression in dorsal midline of hindbrain and spinal cord.
TS24: spinal cord Present Regionally restricted 5H , 5G Expression in dorsal midline of hindbrain and spinal cord.
TS24: forebrain Weak 5H , 5G Expression in forebrain weaker than in more posterior regions. No obvious localization to midline structures.
Specimen 5I: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: medulla oblongata Present Regionally restricted 5I Expression in dorsal and ventral midline
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory