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Gene Expression Data
Assay Details
Assay
Reference: J:154534 Martinez G, et al., Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4794-806
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7660523
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 10A: embryonic day 15.5 (more )
Note: Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Level Pattern Image Note
TS23: lens Absent 10A
TS23: eyelid Present Regionally restricted 10A Expression was detected in the eyelid muscle.
TS23: eye anterior segment Present Regionally restricted 10A Expression was detected in the iris.
Specimen 10B: embryonic day 15.5; Ctnnb1tm1Mmt/Ctnnb1tm1Mmt, Tg(Cryaa-cre)10Mlr/0 (more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Level Pattern Image Note
TS23: lens Present Regionally restricted 10B Expression was detected in numerous cells, which had a highly elongated morphology and resembled myofibroblasts.
TS23: lens fiber Present 10B
Specimen 10D: embryonic day 15.5; Apctm1Tno/Apctm1Tno, Tg(Cryaa-cre)10Mlr/0 (more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Level Pattern Image Note
TS23: lens Present Regionally restricted 10D Expression was detected in numerous cells, which had a highly elongated morphology and resembled myofibroblasts. Immunoreactivity was detected only in the anterior part of the lens.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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