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Gene Expression Data
Assay Details
Assay
Reference: J:195264 Vincentz JW, et al., Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest. PLoS Genet. 2013 Mar;9(3):e1003405
Assay type: RNA in situ
MGI Accession ID: MGI:7643417
Gene symbol: Hand2
Gene name: heart and neural crest derivatives expressed 2
Probe: Hand2 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2C: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Present 2C
Specimen 2D: embryonic day 11.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Present Regionally restricted 2D Conditional ablation of Twist1 produces abnormal cellular aggregates. Expression of Hand2 was detected in the aggregates.
Specimen 8M: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thoracic ganglion Present 8M Expression was detected in the thoracic sympathetic chain ganglia.
Specimen S3I: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Absent S3I
Specimen S3J: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: arch of aorta Absent S3J
Specimen S3K: embryonic day 16.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: cardiac muscle tissue Present Regionally restricted S3K Ectopic expression was detected in the myocardial cuff of the conditional mutant.
Specimen S3L: embryonic day 16.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: arch of aorta Present Regionally restricted S3L Ectopic expression was detected in the aortic arch of the conditional mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory