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Gene Expression Data
Assay Details
Assay
Reference: J:35877 Xiang M, et al., Targeted deletion of the mouse POU domain gene Brn-3a causes selective loss of neurons in the brainstem and trigeminal ganglion, uncoordinated limb movement, and impaired suckling. Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11950-5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7641435
Gene symbol: Pou4f2
Gene name: POU domain, class 4, transcription factor 2
Antibody: anti-Brn-3b
Results
Specimen 3C: embryonic day 20.0 (more )
Structure Cell Type Level Pattern Image Note
TS26: dorsal root ganglion neuron Present 3C Expression in a majority of neurons.
Specimen 3D: embryonic day 20.0; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Structure Cell Type Level Pattern Image Note
TS26: dorsal root ganglion neuron Trace 3D Number of expressing cells greatly reduced compared to wild type. Expressing cells nearly absent.
Specimen 4I: embryonic day 20.0 (more )
Structure Cell Type Level Pattern Image Note
TS26: trigeminal ganglion neuron Present 4I Expression in a majority of neurons.
Specimen 4J: embryonic day 20.0; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Structure Cell Type Level Pattern Image Note
TS26: trigeminal ganglion neuron Trace 4J Density of expressing cells greatly reduced compared to wild type. Expressing cells nearly absent.
Specimen 5C: embryonic day 20.0 (more )
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present 5C Expression only in ganglion cells.
Specimen 5D: embryonic day 20.0; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present 5D Expression unaffected compared to wild type.
Specimen 7C: embryonic day 20.0 (more )
Structure Level Pattern Image Note
TS26: interpeduncular nucleus Present 7C
TS26: superior colliculus Present 7C
TS26: periaqueductal grey Present Regionally restricted 7C Expression in dorsal central grey,
Specimen +/- not shown: embryonic day 20.0; Pou4f1tm1Nat/Pou4f1+ (more )
Structure Level Pattern Image Note
TS26: interpeduncular nucleus Present Expression unchanged compared to wild type. No difference in number or locations of expressing cells.
TS26: superior colliculus Present Expression unchanged compared to wild type. No difference in number or locations of expressing cells.
TS26: periaqueductal grey Present Regionally restricted Expression unchanged compared to wild type. No difference in number or locations of expressing cells.
Specimen 7D: embryonic day 20.0; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Structure Level Pattern Image Note
TS26: interpeduncular nucleus Present 7D Expression unchanged compared to wild type. No difference in number or locations of expressing cells.
TS26: superior colliculus Present 7D Expression unchanged compared to wild type. No difference in number or locations of expressing cells.
TS26: periaqueductal grey Present Regionally restricted 7D Expression unchanged compared to wild type. No difference in number or locations of expressing cells.
Specimen 8E: embryonic day 20.0 (more )
Structure Cell Type Level Pattern Image Note
TS26: inferior olivary nucleus neuron Strong Regionally restricted 8E Strong expression in many neurons in rostral region.
Specimen 8F: embryonic day 20.0; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Structure Cell Type Level Pattern Image Note
TS26: inferior olivary nucleus neuron Present 8F Little change in expression compared to wild type.
Specimen 8G: embryonic day 20.0 (more )
Structure Level Pattern Image Note
TS26: inferior olivary nucleus Present Regionally restricted 8G In caudal region, confined to ventral part. Expression weak or absent in dorsal division.
Specimen 8H: embryonic day 20.0; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Structure Level Pattern Image Note
TS26: inferior olivary nucleus Present 8H Little change in expression compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory