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Gene Expression Data
Assay Details
Assay
Reference: J:70547 Zakany J, et al., Localized and transient transcription of Hox genes suggests a link between patterning and the segmentation clock. Cell. 2001 Jul 27;106(2):207-17
Assay type: RNA in situ
MGI Accession ID: MGI:7640071
Gene symbol: Hoxd3
Gene name: homeobox D3
Probe: Hoxd3 cDNA3
Probe preparation: Antisense RNA
Results
Specimen 5A-1: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: unsegmented mesenchyme Present Regionally restricted 5A-1 Expressed in every nascent somite, with a burst of transcription generating a stripe of expressing cells. Weak and variable expression in the cervical region.
TS12: somite Present 5A-1 Expressed in each of the previously formed somites.
Specimen 5A-2: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: unsegmented mesenchyme Present Regionally restricted 5A-2 Expressed in every nascent somite, with a burst of transcription generating a stripe of expressing cells. Weak and variable expression in the cervical region.
TS12: somite Present 5A-2 Expressed in each of the previously formed somites.
Specimen 5A-3: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: unsegmented mesenchyme Present Regionally restricted 5A-3 Expressed in every nascent somite, with a burst of transcription generating a stripe of expressing cells. Weak and variable expression in the cervical region.
TS12: somite Present 5A-3 Expressed in each of the previously formed somites.
Specimen 5B: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: unsegmented mesenchyme Present 5B Expression in the presegmental plate was evident as late as E9.
Specimen 5C: embryonic day 9.0 (more )
Note: Double labeled: magenta - Hoxd3; red - Mesp2 (assay MGI:7640059). Author mentioned that embryos lacking one copy of Hoxd3 was used. Genotype was not specified.
Structure Level Pattern Image Note
TS14: unsegmented mesenchyme Present Regionally restricted 5C Stripes occurred at the same levels as for Hoxd1, with Mesp2 marking S-I region.
Specimen 7B +/+: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: somite Present 7B-1
TS12: unsegmented mesenchyme Present 7B-1
Specimen 7B -/-: embryonic day 8.0; Rbpjtm1Kyo/Rbpjtm1Kyo (more )
Structure Level Pattern Image Note
TS12: unsegmented mesenchyme Trace 7B-2 Only three out of nine mutant specimens showed detectable expression at the presomitic mesenchyme to somite transition.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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