Probe:	Mm-Piezo1 probe1
Probe preparation:	Antisense DNA
Visualized with:	Fluorescence

Structure	Level	Pattern	Image	Note
TS20: skin	Strong		1a	Prominent expression in skin.
TS20: forebrain	Present	Regionally restricted	1a	Expression in most layers, including ventricular zone, subventricular zone and neuronal layer

Structure	Level	Pattern	Image	Note
TS20: skin	Strong		1b	Prominent expression in skin.
TS20: brain meninges	Strong		1b
TS20: brain blood vessel	Strong		1b	Strong expression in vasculature.
TS20: neocortex	Absent		1b

Structure	Level	Pattern	Image	Note
TS20: brain blood vessel	Strong		1c	Strong expression in vasculature.
TS20: medial ganglionic eminence	Present	Regionally restricted	1c	Expression distinctively located in cells expressing Pdgfra in ventral medial ganglionic eminence (vMGE).
TS20: preoptic area	Present	Regionally restricted	1c	Expression distinctively located in cells expressing Pdgfra in and dorsal preoptic area (dPOA).

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	1d	Co-expressed with Pdgfra confined to ganglionic emininence and ventral cortical regions.

Structure	Level	Pattern	Image	Note
TS22: brain blood vessel	Present		1e
TS22: cerebral cortex	Present	Regionally restricted	1e	Expression in ventral cortical regions.

Structure	Level	Pattern	Image	Note
TS22: brain blood vessel	Strong		1f	Strong expression in vasculature.
TS22: ganglionic eminence	Present		1f

Structure	Level	Pattern	Image	Note
TS24: forebrain	Present	Regionally restricted	2a

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		2b

Structure	Level	Pattern	Image	Note
TS24: piriform cortex	Present	Regionally restricted	2c	More highly expressed in piriform cortex than in dorsal part.

Structure	Level	Pattern	Image	Note
TS24: caudate-putamen	Present	Clusters	2d	Expression in caudate putamen highly clustered in a linear pattern.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	2e

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		2f

Structure	Level	Pattern	Image	Note
TS26: piriform cortex	Present	Regionally restricted	2g	More highly expressed in piriform cortex than in dorsal part.

Structure	Level	Pattern	Image	Note
TS26: caudate-putamen	Present	Clusters	2h	Expression in caudate putamen highly clustered in a linear pattern.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Strong	Regionally restricted	3a	Abundant expression in Nestin-expressing cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex subventricular zone	Present	Regionally restricted	3b	Expression in Nes expressing zones, relatively dispersed throughout ventricular zone and subventricular zone in cortex.
TS20: cerebral cortex ventricular layer	Present	Regionally restricted	3b	Expression in Nes expressing zones, relatively dispersed throughout ventricular zone and subventricular zone in cortex.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	3c	Expression in Nes expressing zones, relatively dispersed throughout ventricular zone and subventricular zone in cganglionic eminence.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Strong	Regionally restricted	3d	Abundant expression in Nestin-expressing cells.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present	Regionally restricted	3e	Expression in Nes expressing zones, relatively dispersed throughout ventricular zone and subventricular zone in cortex.
TS22: cerebral cortex ventricular layer	Present	Regionally restricted	3e	Expression in Nes expressing zones, relatively dispersed throughout ventricular zone and subventricular zone in cortex.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Regionally restricted	3f	Expression in Nes expressing zones, relatively dispersed throughout ventricular zone and subventricular zone in ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS24: forebrain	Weak	Regionally restricted	4a	Expression decreased as brain developed.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Weak		4b

Structure	Level	Pattern	Image	Note
TS24: ganglionic eminence	Weak		4c

Structure	Level	Pattern	Image	Note
TS26: forebrain	Weak	Regionally restricted	4d	Expression decreased as brain developed.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Weak		4e

Structure	Level	Pattern	Image	Note
TS26: ganglionic eminence	Weak		4f

Structure	Level	Pattern	Image	Note
TS20: forebrain	Trace	Regionally restricted	5a	Expression barely detected in Eomes-expressing cells of ventricular zone and subventricular zone at all stages.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex subventricular zone	Trace		5b
TS20: cerebral cortex ventricular layer	Trace		5b

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Trace	Regionally restricted	5c	Expression barely detected in Eomes-expressing cells of ventricular zone and subventricular zone at all stages.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Trace		5d
TS22: cerebral cortex ventricular layer	Trace		5d

Structure	Level	Pattern	Image	Note
TS24: forebrain	Trace	Regionally restricted	6a	Expression barely detected in Eomes-expressing cells of ventricular zone and subventricular zone at all stages.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Trace		6b
TS24: cerebral cortex ventricular layer	Trace		6b

Structure	Level	Pattern	Image	Note
TS26: forebrain	Trace	Regionally restricted	6c	Expression barely detected in Eomes-expressing cells of ventricular zone and subventricular zone at all stages.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Trace		6d
TS26: cerebral cortex ventricular zone	Trace		6d

Structure	Level	Pattern	Image	Note
TS20: forebrain	Trace	Regionally restricted	7a	Minimal expression in Dcx-expressing cells. at E12.5.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Trace		7b

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Trace		7c

Structure	Level	Pattern	Image	Note
TS22: forebrain	Trace	Regionally restricted	7d	Minimal expression in Dcx-expressing cells. at E14.5.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Trace		7e

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Trace		7f

Structure	Level	Pattern	Image	Note
TS24: forebrain	Present	Regionally restricted	8a

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	8b

Structure	Level	Pattern	Image	Note
TS24: piriform cortex	Strong	Widespread	8c	Expression markedly increased and widely expressed throughout piriform cortex at E14.5, overlapping with Dcx-expressing cells.

Structure	Level	Pattern	Image	Note
TS24: caudate-putamen	Present		8d

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present	Regionally restricted	8e

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	8f

Structure	Level	Pattern	Image	Note
TS26: piriform cortex	Strong	Widespread	8g	Expression markedly increased and widely expressed throughout piriform cortex at E16.5, overlapping with Dcx-expressing cells.

Structure	Level	Pattern	Image	Note
TS26: caudate-putamen	Present		8h