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Gene Expression Data
Assay Details
Assay
Reference: J:347585 Leon NY, et al., Y chromosome damage underlies testicular abnormalities in ATR-X syndrome. iScience. 2024 May 17;27(5):109629
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7628675
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phospho-histone H2A.X (20E3) (CST;#9718)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: green - H2ax; red - Gata4 (assay MGI:7628670).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 3A Control (merged) , 3A Control g-H2AX Prominent expression in 10% of Sertoli cells with Gata4 foci: 4.1% localized and 6.2% widespread.
Specimen 3B ScAttrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - Gata4 (assay MGI:7628670).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 3A ScAtrxKO g-H2AX , 3A ScAtrxKO (merged) Widespread expresssion in Sertoli cells with regular-sized Gata4 foci (5.8%); localized expression in 43.7%. Expresssion in all G2/M arrested Sertoli cells with giant Gata4 foci (51% localized and 48.3% localized).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory