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Gene Expression Data
Assay Details
Assay
Reference: J:236179 Bowman CE, et al., Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series. Mol Cell Biol. 2016 Aug 1;36(15):2089-104
Assay type: RT-PCR
MGI Accession ID: MGI:7628356
Gene symbol: Pck1
Gene name: phosphoenolpyruvate carboxykinase 1, cytosolic
Probe: Pck1-pF, Pck1-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to 4 reference genes (18S rRNA, Rpl22, beta-actin, and Gapdh) and shown relative to wild type.
Results Image: 7F Pck1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
WT E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 * SJL Not Specified
KI/+ E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1+Not Specified
D/+ E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 Mpc1tm1.2Mwol/Mpc1+Not Specified
KI/KI E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1tm1.1MwolNot Specified
D/KI E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1tm1.2MwolNot Specified
KI/KI; Cre E17.5 TS25: liver Present 0.01 µg; total RNA involves: C57BL/6 * SJL Mpc1tm1.1Mwol/Mpc1tm1.1Mwol, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Notes:
(a) In liver, the greater suppression of Mpc1 expression resulted in a concomitant increase in the expression of Pck1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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