Gene Expression Data
Assay Details
Assay
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Reference:
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J:345818
Petrova RS, et al., Verification of the gene and protein expression of the aquaglyceroporin AQP3 in the mammalian lens. Exp Eye Res. 2024 Mar;240:109828
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7628218
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Gene symbol:
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Mip
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Gene name:
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major intrinsic protein of lens fiber
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Antibody:
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anti-AQP0
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
E10 not shown:
embryonic day 10.0
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
E10 not shown:
(close )
Genetic Background:
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FVB/N
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: lens vesicle |
Present |
Regionally restricted |
|
Membranous expression in cortex
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Specimen
E11 not shown:
embryonic day 11.0
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
E11 not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Formalin
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: lens vesicle |
Present |
Regionally restricted |
|
Membranous expression in cortex
|
Specimen
E16 not shown:
embryonic day 16.0
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
E16 not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 16.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lens core |
Present |
Regionally restricted |
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Membranous expression in cortex and core.
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TS24: lens cortex |
Present |
Regionally restricted |
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Membranous expression in cortex and core.
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Specimen
P3 not shown:
postnatal day 3
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
P3 not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lens core |
Present |
Regionally restricted |
|
Membranous expression in cortex and core.
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TS27: lens cortex |
Present |
Regionally restricted |
|
Membranous expression in cortex and core.
|
Specimen
P6 not shown:
postnatal day 6
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
P6 not shown:
(close )
Genetic Background:
|
C57BL/6
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Age:
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postnatal day 6
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens cortex |
Present |
Regionally restricted |
|
Membranous expression in cortex.
|
TS28: lens core |
Present |
Regionally restricted |
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Truncated expression in core.
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Specimen
P15 not shown:
postnatal day 15
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
P15 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 15
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens cortex |
Present |
Regionally restricted |
|
Membranous expression in cortex.
|
TS28: lens core |
Present |
Regionally restricted |
|
Truncated expression in core.
|
Specimen
7L:
postnatal adult
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
7L:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
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Note:
|
Double labeled: green - Mip; red - WGA.
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Specimen
7M:
postnatal adult
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
7M:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mip; red - WGA.
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Specimen
7N:
postnatal adult
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
7N:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens inner cortex |
Present |
Regionally restricted |
7N
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Membranous expression in inner cortex.
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Specimen
7O:
postnatal adult
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
7O:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mip; red - WGA.
|
Specimen
7P:
postnatal adult
(more )
Note: Double labeled: green - Mip; red - WGA.
Specimen
7P:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mip; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens core |
Absent |
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7P
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Expression absent in deeper regions of lens.
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