Probe:	SIP1 3' probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		2a
TS15: notochord	Weak		2a
TS15: yolk sac	Present		2a

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Strong		2b
TS17: dorsal root ganglion	Strong		2b
TS17: myotome	Strong		2b
TS17: telencephalon	Strong		2b
TS17: trigeminal ganglion	Strong		2b
TS17: neural tube	Strong		2b
TS17: blood vessel	Present		2b
TS17: dorsal aorta	Present		2b
TS17: heart	Present		2b
TS17: liver	Present		2b
TS17: limb bud	Absent		2b

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Strong		2c
TS19: neural tube	Strong		2c
TS19: liver	Present		2c
TS19: lung	Present		2c

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		2d
TS20: hindbrain ventricular layer	Strong		2d
TS20: skeletal muscle	Strong		2d
TS20: spinal cord lumen	Strong	Regionally restricted	2d	Expression was mostly observed in the ependymal layer of the neural tube.
TS20: liver	Weak		2d	Diminished to a low/undetectable level.

Structure	Level	Pattern	Image	Note
TS20: endocardial lining	Weak	Regionally restricted	2e	Expressed in the endocardium of the developing heart values.
TS20: heart valve primordium	Weak	Regionally restricted	2e	Expressed in the endocardium of the developing heart values.
TS20: blood vessel	Present		2e
TS20: dorsal aorta	Present		2e
TS20: umbilical cord mesenchyme	Present		2e
TS20: ventricle cardiac muscle	Absent		2e

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		2f
TS22: choroid plexus	Present		2f
TS22: corpus striatum	Present		2f
TS22: retina	Present		2f
TS22: thalamus	Present		2f
TS22: spinal cord	Present	Regionally restricted	2f	Expressed in the dorsal region.
TS22: facial muscle	Strong	Regionally restricted	2f	Expressed in the frontonasal mass, muscles of the jaw and nasal sinuses.
TS22: eye mesenchyme	Strong	Regionally restricted	2f	Expressed in the mesenchyme around the eyes.
TS22: face mesenchyme	Strong	Regionally restricted	2f	Expressed in mesenchyme around the whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present		2g
TS22: gonad	Present		2g
TS22: gut	Present		2g
TS22: metanephros	Present		2g
TS22: spinal cord	Present		2g
TS22: alimentary system smooth muscle	Strong		2g
TS22: skeletal muscle	Strong		2g
TS22: jaw	Present	Regionally restricted	2g	Expressed in the distal region.

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Strong		2h	More robust expression than Zeb1.
TS19: dorsal root ganglion	Strong		2h	More robust expression than Zeb1.
TS19: trigeminal ganglion	Strong		2h	More robust expression than Zeb1.
TS19: hindbrain	Strong		2h	Expression overlapped with Zeb1.
TS19: myotome	Strong		2h	Expression overlapped with Zeb1.
TS19: neural tube	Strong		2h	Expression overlapped with Zeb1.
TS19: telencephalon	Strong		2h	Expression overlapped with Zeb1.
TS19: heart	Present		2h	Expression was not overlapped with Zeb1.
TS19: liver	Present		2h	Expression was not overlapped with Zeb1.
TS19: lung	Present		2h	Expression was not overlapped with Zeb1.
TS19: face	Present	Regionally restricted	2h	Expressed in the frontonasal mass, not overlapped with Zeb1.
TS19: jaw primordium	Present	Regionally restricted	2h	Expressed in the distal region, not overlapped with Zeb1.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present

Structure	Level	Pattern	Image	Note
TS17: liver	Present

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present
TS22: cochlear ganglion	Present
TS22: brain nucleus	Present	Regionally restricted		Expressed in the trigeminal nucleus.
TS22: brainstem nucleus	Present	Regionally restricted		Expressed in the inferior olive.

Structure	Level	Pattern	Image	Note
TS27: brain	Present