Cdkn1b Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:242186 Hsieh CP, et al., Deficits in cerebellar granule cell development and social interactions in CD47 knockout mice. Dev Neurobiol. 2015 May;75(5):463-84
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7627159
Gene symbol:	Cdkn1b
Gene name:	cyclin dependent kinase inhibitor 1B

Antibody:	anti-p27
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 4A WT: postnatal day 8 (more

)
Note: Double labeled: green - Cdkn1b; red - BrdU.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	Regionally restricted	4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum molecular layer	cerebellar granule cell	Present	Regionally restricted	4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule II	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule III	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule IV	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule IX	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule V	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule VIII	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule X	cerebellar granule cell	Present		4A WT	Expression is in postmitotic cerebellar granule cells.

Specimen 4A CD47^+/-: postnatal day 8; Cd47^tm1Fpl/Cd47⁺ (more

)
Note: Double labeled: green - Cdkn1b; red - BrdU.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	Regionally restricted	4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule V	cerebellar granule cell	Present		4A CD47^+/-	In this heterozygote there is a significant reduction in the percentage of positive cells.
TS28: cerebellum vermis lobule II	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule III	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule IV	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule IX	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule VIII	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.
TS28: cerebellum vermis lobule X	cerebellar granule cell	Present		4A CD47^+/-	Expression is in postmitotic cerebellar granule cells.

Specimen 4A CD47^-/-: postnatal day 8; Cd47^tm1Fpl/Cd47^tm1Fpl (more

)
Note: Double labeled: green - Cdkn1b; red - BrdU.

Structure	Cell Type	Level	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Present	4A CD47^-/-	In this mutant there is a significant reduction in the percentage of positive postmitotic cerebellar granule cells in most lobules.
TS28: cerebellum vermis lobule IV	cerebellar granule cell	Present	4A CD47^-/-	In this mutant there is a significant reduction in the percentage of positive cells.
TS28: cerebellum vermis lobule V	cerebellar granule cell	Present	4A CD47^-/-	In this mutant there is a significant reduction in the percentage of positive cells.
TS28: cerebellum vermis lobule VI	cerebellar granule cell	Present	4A CD47^-/-	In this mutant there is a significant reduction in the percentage of positive cells.
TS28: cerebellum vermis lobule VII	cerebellar granule cell	Present	4A CD47^-/-	In this mutant there is a significant reduction in the percentage of positive cells.
TS28: cerebellum vermis lobule VIII	cerebellar granule cell	Present	4A CD47^-/-	In this mutant there is a significant reduction in the percentage of positive cells.
TS28: cerebellum vermis lobule X	cerebellar granule cell	Present	4A CD47^-/-	In this mutant there is a significant reduction in the percentage of positive cells.
TS28: cerebellum vermis lobule II	cerebellar granule cell	Present	4A CD47^-/-	Expression is in postmitotic cerebellar granule cells and is similar to wild-type.
TS28: cerebellum vermis lobule III	cerebellar granule cell	Present	4A CD47^-/-	Expression is in postmitotic cerebellar granule cells and is similar to wild-type.
TS28: cerebellum vermis lobule IX	cerebellar granule cell	Present	4A CD47^-/-	Expression is in postmitotic cerebellar granule cells and is similar to wild-type.

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