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Gene Expression Data
Assay Details
Assay
Reference: J:308932 Bulut-Karslioglu A, et al., Chd1 protects genome integrity at promoters to sustain hypertranscription in embryonic stem cells. Nat Commun. 2021 Aug 11;12(1):4859
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7627082
Gene symbol: H2ax
Gene name: H2A.X variant histone
Results
Specimen 2c Control E5.5: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS7: embryo Present 2c Control E5.5 Expression was mainly localized to the nucleolus along with a diffuse nuclear pattern.
Specimen 2c Chd1 KO E5.5: embryonic day 5.5; Chd1tm1b(KOMP)Rsan/Chd1tm1b(KOMP)Rsan (more )
Structure Level Pattern Image Note
TS7: embryo Present 2c Chd1 KO E5.5 Chd1 KO embryos entirely lack the gammaH2A.X signal, while global H2A.X is retained.
Specimen 2c Control E6.5: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: embryo Present 2c Control E6.5 Expression was mainly localized to the nucleolus along with a diffuse nuclear pattern.
Specimen 2c Chd1 KO E6.5: embryonic day 6.5; Chd1tm1b(KOMP)Rsan/Chd1tm1b(KOMP)Rsan (more )
Structure Level Pattern Image Note
TS9: embryo Present 2c Chd1 KO E6.5 Chd1 KO embryos entirely lack the gammaH2A.X signal, while global H2A.X is retained.
Specimen 2d: embryonic day 5.5 (more )
Note: Double labeled: green - H2ax; red - Ncl (assay MGI:7627083).
Structure Level Pattern Image Note
TS7: epiblast Present 2d left , 2d right Expression was mainly localized to the nucleolus along with a diffuse nuclear pattern.
Specimen 2e Control: embryonic day 6.5 (more )
Note: Double labeled: red - H2ax; green - RNA Pol II S2p.
Structure Level Pattern Image Note
TS8: epiblast Present 2e Control Expression was mainly localized to the nucleolus along with a diffuse nuclear pattern.
Specimen 2e Chd1 KO: embryonic day 6.5; Chd1tm1b(KOMP)Rsan/Chd1tm1b(KOMP)Rsan (more )
Note: Double labeled: red - H2ax; green - RNA Pol II S2p.
Structure Level Pattern Image Note
TS8: epiblast Present 2e Chd1 KO Chd1 KO embryos entirely lack the gammaH2A.X signal, while global H2A.X is retained.
Specimen S2a: embryonic day 5.5 (more )
Note: Double labeled: green - H2ax; red - Ncl (assay MGI:7627083).
Structure Level Pattern Image Note
TS7: embryo Present S2a Merge , S2a Merge inset left , S2a Merge inset right , S2a gammaH2A.X Gamma-H2A.X co-localized with Ncl.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory