Chd1^{tm1b(KOMP)Rsan}
Targeted Allele Detail

Summary

• Symbol: Chd1^{tm1b(KOMP)Rsan}
• Name: chromodomain helicase DNA binding protein 1; targeted mutation 1b, Miguel Ramalho-Santos
• MGI ID: MGI:5637386
• Gene: Chd1 Location: Chr17:15925229-15992872 bp, + strand Genetic Position: Chr17, 8.95 cM
• Alliance: Chd1^{tm1b(KOMP)Rsan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217689
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 15961936 of Chromosome 17 upstream of the critical exon 16 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 16 at position 15962829. The critical exon 16 is thus flanked by loxP sites. A knock-out allele was created by cre recombinase expression in ES cells carrying this allele. (J:217689)

Expression

In Mice Carrying this Mutation:	15 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chd1 Mutation: 93 strains or lines available

References

• Original: J:217689 Guzman-Ayala M, et al., Chd1 is essential for the high transcriptional output and rapid growth of the mouse epiblast. Development. 2015 Jan 1;142(1):118-27
• All: 3 reference(s)