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Gene Expression Data
Assay Details
Assay
Reference: J:235672 Nam H, et al., Identification of STAM1 as a novel effector of ventral projection of spinal motor neurons. Development. 2016 Jul 1;143(13):2334-43
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7623980
Gene symbol: Stam
Gene name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Antibody: anti-STAM1
Results
Specimen 2A: embryonic day 9.5 (more )
Note: Double labeled: green - Stam; red - Mnx1 (assay MGI:7623979).
Structure Cell Type Level Pattern Image Note
TS15: future spinal cord motor neuron Present 2A STAM1/Hb9 (right) , 2A STAM1 (left) , 2A STAM1/Hb9 (left) , 2A STAM1 (right) Expression in developing motor neurons. beginning around E9.5.
Specimen 2B: embryonic day 10.5 (more )
Note: Double labeled: green - Stam; red - Isl1 (assay MGI:7623981) and Mnx1 (assay MGI:7623979).
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord motor neuron Strong 2B STAM1 (right) , 2B STAM1/Hb9 , 2B STAM1/Isl1 , 2B STAM1 (left) Expression strongly induced in motor neurons at E10.5.
TS17: dorsal root ganglion Present 2B STAM1/Hb9 , 2B STAM1/Isl1 , 2B STAM1 (left) , 2B STAM1 (right)
Specimen 2C: embryonic day 10.5 (more )
Note: Double labeled: green - Stam; red - Isl1 (assay MGI:7623981).
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord motor neuron Present Regionally restricted 2C STAM1/Isl1 , 2C STAM1 Expression in motor neurons mainly loalized in cytoplasm and axons and exhibited a punctate pattern.
Specimen E11.5 not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: future spinal cord Strong Expression in neuronal zone but remained strongest in motor neurons.
TS19: dorsal root ganglion Present
Specimen 3C WT: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: spinal cord motor neuron Present 3C WT STAM1 (left) , 3C WT STAM1 (right)
TS20: dorsal root ganglion Present 3C WT STAM1 (right) , 3C WT STAM1 (left)
Specimen 3C dKO: embryonic day 12.5; Mnx1tm1Jhk/Mnx1tm1Jhk, Isl2tm2Slp/Isl2tm2Slp (more )
Structure Cell Type Level Pattern Image Note
TS20: spinal cord motor neuron Weak 3C dKO STAM1 (right) , 3C dKO STAM1 (left) Expression in postmitoric motor neurons drastically reduced (66%) compared to control. Expression in dorsal spinal cord relatively intact (95% compared to control.
TS20: dorsal root ganglion Weak 3C dKO STAM1 (right) , 3C dKO STAM1 (left) Expression in dorsal root ganglion also slightly dampened (83%) compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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