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Gene Expression Data
Assay Details
Assay
Reference: J:302851 Greschik H, et al., The histone code reader Spin1 controls skeletal muscle development. Cell Death Dis. 2017 Nov 23;8(11):e3173
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7622055
Gene symbol: Myh2
Gene name: myosin, heavy polypeptide 2, skeletal muscle, adult
Antibody: clone MY-32 (M4276)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Assay notes: The specificity of the antibody used was not detailed; all family members have been annotated.
Results
Specimen 1c E15: embryonic day 15.0 (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Spin1 (assay MGI:7621392).
Structure Cell Type Level Pattern Image Note
TS23: leg muscle fetal and neonatal skeletal muscle fiber Present 1c E15 middle , 1c E15 right Expressed in myofibers.
Specimen 1c P0: postnatal day 0 (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Spin1 (assay MGI:7621392).
Structure Cell Type Level Pattern Image Note
TS27: leg muscle fetal and neonatal skeletal muscle fiber Present 1c P0 right , 1c P0 middle Expressed in myofibers.
Specimen 1e: embryonic day 15.0; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2 and Myh1 and Myh4; green - Spin1 (assay MGI:7621392).
Structure Level Pattern Image Note
TS23: leg muscle Present 1e middle , 1e right
Specimen 3c Ctrl-1: embryonic day 16.5; Spin1tm1.1Rosc/Spin1+ (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd1 (assay MGI:7621554).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c Ctrl-1
Specimen 3c Ctrl-2: embryonic day 16.5; Spin1tm1.1Rosc/Spin1+ (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd1 (assay MGI:7621554).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c Ctrl-2
Specimen 3c Ctrl-3: embryonic day 16.5; Spin1tm1.1Rosc/Spin1+ (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd2 (assay MGI:7621556).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c Ctrl-3
Specimen 3c Ctrl-4: embryonic day 16.5; Spin1tm1.1Rosc/Spin1+ (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd2 (assay MGI:7621556).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c Ctrl-4
Specimen 3c M5-1: embryonic day 16.5; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd1 (assay MGI:7621554).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c M5-1
Specimen 3c M5-2: embryonic day 16.5; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd1 (assay MGI:7621554).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c M5-2
Specimen 3c M5-3: embryonic day 16.5; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd2 (assay MGI:7621556).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c M5-3
Specimen 3c M5-4: embryonic day 16.5; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd2 (assay MGI:7621556).
Structure Cell Type Level Pattern Image Note
TS24: tibialis anterior fetal and neonatal skeletal muscle fiber Present 3c M5-4
Specimen S3a Ctrl left: embryonic day 15.0 (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd1 (assay MGI:7621554).
Structure Cell Type Level Pattern Image Note
TS23: tibialis anterior fetal and neonatal skeletal muscle fiber Present S3a Ctrl-2 , S3a Ctrl-1
Specimen S3a Ctrl right/3b: embryonic day 15.0 (more )
Note: Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd2 (assay MGI:7621556).
Structure Cell Type Level Pattern Image Note
TS23: tibialis anterior fetal and neonatal skeletal muscle fiber Present S3a Ctrl-4 , S3b merge , S3a Ctrl-3
Specimen S3a M5 left: embryonic day 15.0; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd1 (assay MGI:7621554).
Structure Cell Type Level Pattern Image Note
TS23: tibialis anterior fetal and neonatal skeletal muscle fiber Present S3a M5-2 , S3a M5-1
Specimen S3a M5 right: embryonic day 15.0; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2 and Myh1 and Myh4; green - Ankrd2 (assay MGI:7621556).
Structure Cell Type Level Pattern Image Note
TS23: tibialis anterior fetal and neonatal skeletal muscle fiber Present S3a M5-3 , S3a M5-4
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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