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Gene Expression Data
Assay Details
Assay
Reference: J:302851 Greschik H, et al., The histone code reader Spin1 controls skeletal muscle development. Cell Death Dis. 2017 Nov 23;8(11):e3173
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7621566
Gene symbol: Myh2
Gene name: myosin, heavy polypeptide 2, skeletal muscle, adult
Antibody: anti-MycIIa (SC-71)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 6f Ctrl-white: postnatal week 15 (more )
Note: Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIa muscle cell Present 6f IIa-IIx-1
Specimen 6f M5-white: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIa muscle cell Ambiguous 6f IIa-IIx-2 Rarely observed type IIa fibers in some of the mutants.
Specimen 6f Ctrl-red: postnatal week 15 (more )
Note: Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIa muscle cell Present 6f IIa-IIx-3 Mainly type IIa and IIx fibers in the red portion (primarily oxidative).
Specimen 6f M5-red: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Level Pattern Image Note
TS28: tibialis anterior Ambiguous 6f IIa-IIx-4 Rarely observed type IIa fibers in some of the mutants.
Specimen S5e Ctrl SOL: postnatal week 15 (more )
Note: Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: soleus type IIa muscle cell Present S5e IIa-IIx-1
Specimen S5e M5 SOL: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: soleus type IIa muscle cell Present S5e IIa-IIx-2
Specimen S5e Ctrl PL: postnatal week 15 (more )
Note: Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: plantaris type IIa muscle cell Present S5e IIa-IIx-3
Specimen S5e M5 PL: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: plantaris type IIa muscle cell Present S5e IIa-IIx-4
Specimen S5e M5 TA: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: red - Myh2; green - Myh1 (assay MGI:7621560).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIa muscle cell Present S5e IIa-IIx-5
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory