About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:302851 Greschik H, et al., The histone code reader Spin1 controls skeletal muscle development. Cell Death Dis. 2017 Nov 23;8(11):e3173
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7621565
Gene symbol: Myh4
Gene name: myosin, heavy polypeptide 4, skeletal muscle
Antibody: anti-MycIIb (BF-F3)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6f Ctrl-white: postnatal week 15 (more )
Note: Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIb muscle cell Present 6f I-IIb-1 Mainly type IIb fibers in the white portion (primarily glycolytic).
Specimen 6f M5-white: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIb muscle cell Present 6f I-IIb-2 Defective fibers could be identified by irregular fiber size, shape, or NADH staining.
Specimen 6f Ctrl-red: postnatal week 15 (more )
Note: Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIb muscle cell Present 6f I-IIb-3 Type IIb fibers were not the major fibers in the red portion (primarily oxidative).
Specimen 6f M5-red: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIb muscle cell Present 6f I-IIb-4 Defective fibers could be identified by irregular fiber size, shape, or NADH staining.
Specimen S5e Ctrl SOL: postnatal week 15 (more )
Note: Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Level Pattern Image Note
TS28: soleus Present S5e I-IIb-1 Type IIb fibers were not the major fibers.
Specimen S5e M5 SOL: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Level Pattern Image Note
TS28: soleus Present S5e I-IIb-2 Type IIb fibers were not the major fibers.
Specimen S5e Ctrl PL: postnatal week 15 (more )
Note: Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Cell Type Level Pattern Image Note
TS28: plantaris type IIb muscle cell Present S5e I-IIb-3 Mainly type IIb fibers.
Specimen S5e M5 PL: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Level Pattern Image Note
TS28: plantaris Present S5e I-IIb-4 Mainly type IIb fibers.
Specimen S5e M5 TA: postnatal week 15; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: cyan - Myh4; purple - Myh7 (assay MGI:7621567).
Structure Cell Type Level Pattern Image Note
TS28: tibialis anterior type IIb muscle cell Present S5e I-IIb-5 Mainly type IIb fibers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory