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Gene Expression Data
Assay Details
Assay
Reference: J:302851 Greschik H, et al., The histone code reader Spin1 controls skeletal muscle development. Cell Death Dis. 2017 Nov 23;8(11):e3173
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7621392
Gene symbol: Spin1
Gene name: spindlin 1
Antibody: anti-SPIN1(2)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1b E15: embryonic day 15.0 (more )
Note: Double labeled: green - Spin1; red - Pax7 (assay MGI:7621543).
Structure Cell Type Level Pattern Image Note
TS23: leg muscle skeletal muscle satellite myogenic cell Strong 1b E15 middle , 1b E15 left Coexpressed with Pax7 in myoblast precursors.
Specimen 1b P0: postnatal day 0 (more )
Note: Double labeled: green - Spin1; red - Pax7 (assay MGI:7621543).
Structure Cell Type Level Pattern Image Note
TS27: leg muscle skeletal muscle satellite myogenic cell Strong 1b P0 left , 1b P0 middle Coexpressed with Pax7 in myoblast precursors.
Specimen 1c E15: embryonic day 15.0 (more )
Note: Double labeled: green - Spin1; red - Myh1 (assay MGI:7621553) and Myh2 (assay MGI:7622055) (assay MGI:7621553) and Myh4 (assay MGI:7622056).
Structure Cell Type Level Pattern Image Note
TS23: leg muscle fetal and neonatal skeletal muscle fiber Weak 1c E15 right , 1c E15 middle , 1c E15 left Weaker staining in nuclei of myofibers was shown.
Specimen 1c P0: postnatal day 0 (more )
Note: Double labeled: green - Spin1; red - Myh1 (assay MGI:7621553) and Myh2 (assay MGI:7622055) (assay MGI:7621553) and Myh4 (assay MGI:7622056).
Structure Cell Type Level Pattern Image Note
TS27: leg muscle fetal and neonatal skeletal muscle fiber Present 1c P0 left , 1c P0 right , 1c P0 middle Expression was undetectable in some nuclei of myofibers.
Specimen 1d: embryonic day 15.0; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: green - Spin1; red - Pax7 (assay MGI:7621543).
Structure Cell Type Level Pattern Image Note
TS23: leg muscle Present 1d left , 1d middle
TS23: leg muscle skeletal muscle satellite myogenic cell Absent 1d middle , 1d left No coexpression with Pax7.
Specimen 1e: embryonic day 15.0; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: green - Spin1; red - Myh1 (assay MGI:7621553) and Myh2 (assay MGI:7622055) (assay MGI:7621553) and Myh4 (assay MGI:7622056).
Structure Cell Type Level Pattern Image Note
TS23: leg muscle Present 1e left , 1e right , 1e middle No expression in MHC+ myofibers.
TS23: leg muscle fetal and neonatal skeletal muscle fiber Absent 1e left , 1e middle
Specimen S1e: embryonic day 17.5; Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: green - Spin1; red - Tcf7l2 (assay MGI:7621571).
Structure Cell Type Level Pattern Image Note
TS25: leg muscle skeletal muscle fibroblast Present S1e left , S1e middle Coexpressed with Tcf4/Tcf7l2 in fibroblasts.
Specimen S1f Ctrl: postnatal day 0 (more )
Note: Double labeled: green - Spin1; white - GFP-NLS-lacZ/GNZ reporter.
Structure Cell Type Level Pattern Image Note
TS27: leg muscle skeletal muscle satellite myogenic cell Present S1f Ctrl left Coexpressed with Pax7.
Specimen S1f M5: postnatal day 0; Gt(ROSA)26Sortm1Joe/Gt(ROSA)26Sor+, Myf5tm3(cre)Sor/Myf5+, Spin1tm1.1Rosc/Spin1tm1.1Rosc (more )
Note: Conditional mutant. Double labeled: green - Spin1; white - GFP-NLS-lacZ/GNZ reporter.
Structure Cell Type Level Pattern Image Note
TS27: leg muscle skeletal muscle satellite myogenic cell Absent S1f M5 left No coexpression with Pax7.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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