Hoxb4 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:199353 Fleury M, et al., Mpl receptor defect leads to earlier appearance of hematopoietic cells/hematopoietic stem cells in the Aorta-Gonad-Mesonephros region, with increased apoptosis. Int J Dev Biol. 2010;54(6-7):1067-74
Assay type:	RT-PCR
MGI Accession ID:	MGI:7620482
Gene symbol:	Hoxb4
Gene name:	homeobox B4

Probe:	Hoxb4-pF, Hoxb4-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

Image: 4A HoxB4

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
C57Bl/6	E10.5	TS17: aorta-gonad-mesonephros	Present	1 µg; total RNA	C57BL/6		Not Specified
Mlp^-/-	E10.5	TS17: aorta-gonad-mesonephros	Present (a)	1 µg; total RNA	involves: 129/Sv * C57BL/6	Mpl^tm1Fjs/Mpl^tm1Fjs	Not Specified

Notes:
(a) There was a 1.2 fold reduction in expression in this mutant compared to wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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