Mpl^tm1Fjs
Targeted Allele Detail

Summary

• Symbol: Mpl^tm1Fjs
• Name: myeloproliferative leukemia virus oncogene; targeted mutation 1, Frederic J de Sauvage
• MGI ID: MGI:2686901
• Synonyms: c-Mpl^-, c-Mpl delta60
• Gene: Mpl Location: Chr4:118299612-118314710 bp, - strand Genetic Position: Chr4, 54.61 cM, cytoband D
• Alliance: Mpl^tm1Fjs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87416
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 11 and 12, which includes the sequence for the intracellular region, were replaced with a complex sequence including a cDNA fragment encoding only the first half of the cytoplasmic signaling domain, an SV40 polyadenylation site, and a floxed PGK-neomycin cassette. Western blot analysis demonstrated that the resulting protein product had been modified to a size consistent with a 60 amino acid deletion. (J:87416)

Disease models

Expression

In Mice Carrying this Mutation:

7 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mpl Mutation: 46 strains or lines available

References

• Original: J:87416 Luoh SM, et al., Role of the distal half of the c-Mpl intracellular domain in control of platelet production by thrombopoietin in vivo. Mol Cell Biol. 2000 Jan;20(2):507-15
• All: 12 reference(s)