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Gene Expression Data
Assay Details
Assay
Reference: J:192361 Srinivasan K, et al., A network of genetic repression and derepression specifies projection fates in the developing neocortex. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19071-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7620298
Gene symbol: Tbr1
Gene name: T-box brain transcription factor 1
Antibody: anti-Tbr1
Results
Specimen 5A: postnatal day 4; Satb2tm1Rug/Satb2tm2Rug (more )
Note: Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer VI cerebral cortex neuron Strong 5A
TS28: cortical layer V cerebral cortex neuron Moderate 5A
TS28: neocortex layer cerebral cortex neuron Weak Regionally restricted 5A A small fraction of cells in the upper layers were also immunopositive.
Specimen 5B: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Satb2tm1Rug/Satb2tm1Rug (more )
Note: Conditional mutant. Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V cerebral cortex neuron Weak 5B
TS28: cortical layer VI cerebral cortex neuron Weak 5B
TS28: neocortex layer cerebral cortex neuron Weak Regionally restricted 5B Satb2 conditional knockouts show reduced Tbr1 in layers 6 and 5, and a loss of Tbr1 in the upper layers.
Specimen 5C: postnatal day 0; Bcl11btm1Jpk/Bcl11btm1Jpk (more )
Note: Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS27: cortical layer V cerebral cortex neuron Strong 5C A significant increase in the number of immunopositive layer 5 neurons was observed.
TS27: cortical layer VI cerebral cortex neuron Strong 5C
Specimen 5D: postnatal day 0; Bcl11btm1Jpk/Bcl11btm1Jpk, Emx1tm1(cre)Krj/Emx1+, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant. Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS27: cortical layer V cerebral cortex neuron Weak 5D
Specimen 5E: postnatal day 4; Fezf2tm1Skm/Fezf2tm1Skm (more )
Note: Double labeled: green - Tbr1; red - Bcl11b (assay MGI:7620290).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V cerebral cortex neuron Strong 5E An increase in expression was observed in the mutant.
TS28: cortical layer VI cerebral cortex neuron Strong 5E Expression in layer VI was not changed in the mutant.
Specimen 5F: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant. Double labeled: green - Tbr1; red - Bcl11b (assay MGI:7620290).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V cerebral cortex neuron Present 5F Expression level was not restored to control levels in this mutant.
Specimen S4A: postnatal; Bcl11btm1Jpk/Bcl11btm1Jpk (more )
Note: Triple labeled: blue - Tbr1; red - Fezf2 (assay MGI:7620477); green - Satb2 (assay MGI:7620478).
Structure Level Pattern Image Note
TS28: cortical layer V Present S4A Increased immunostaining was observed in the mutant.
TS28: cortical layer VI Present S4A Increased immunostaining was observed in the mutant.
Specimen S4E: postnatal; Bcl11btm1Jpk/Bcl11btm1Jpk, Fezf2tm1Skm/Fezf2tm1Skm (more )
Structure Level Pattern Image Note
TS28: cortical layer VI Present S4E Normal immunostaining was observed in layer 6.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory