About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:192361 Srinivasan K, et al., A network of genetic repression and derepression specifies projection fates in the developing neocortex. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19071-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7620298
Gene symbol: Tbr1
Gene name: T-box brain transcription factor 1
Antibody: anti-Tbr1
Results
Specimen 5A: postnatal day 4; Satb2tm1Rug/Satb2tm2Rug (more )
Note: Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer VI cerebral cortex neuron Strong 5A
TS28: cortical layer V cerebral cortex neuron Moderate 5A
TS28: neocortex layer cerebral cortex neuron Weak Regionally restricted 5A A small fraction of cells in the upper layers were also immunopositive.
Specimen 5B: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Satb2tm1Rug/Satb2tm1Rug (more )
Note: Conditional mutant. Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V cerebral cortex neuron Weak 5B
TS28: cortical layer VI cerebral cortex neuron Weak 5B
TS28: neocortex layer cerebral cortex neuron Weak Regionally restricted 5B Satb2 conditional knockouts show reduced Tbr1 in layers 6 and 5, and a loss of Tbr1 in the upper layers.
Specimen 5C: postnatal day 0; Bcl11btm1Jpk/Bcl11btm1Jpk (more )
Note: Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS27: cortical layer V cerebral cortex neuron Strong 5C A significant increase in the number of immunopositive layer 5 neurons was observed.
TS27: cortical layer VI cerebral cortex neuron Strong 5C
Specimen 5D: postnatal day 0; Bcl11btm1Jpk/Bcl11btm1Jpk, Emx1tm1(cre)Krj/Emx1+, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant. Double labeled: green - Tbr1; red - Bhlhe22 (assay MGI:7620291).
Structure Cell Type Level Pattern Image Note
TS27: cortical layer V cerebral cortex neuron Weak 5D
Specimen 5E: postnatal day 4; Fezf2tm1Skm/Fezf2tm1Skm (more )
Note: Double labeled: green - Tbr1; red - Bcl11b (assay MGI:7620290).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V cerebral cortex neuron Strong 5E An increase in expression was observed in the mutant.
TS28: cortical layer VI cerebral cortex neuron Strong 5E Expression in layer VI was not changed in the mutant.
Specimen 5F: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant. Double labeled: green - Tbr1; red - Bcl11b (assay MGI:7620290).
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V cerebral cortex neuron Present 5F Expression level was not restored to control levels in this mutant.
Specimen S4A: postnatal; Bcl11btm1Jpk/Bcl11btm1Jpk (more )
Note: Triple labeled: blue - Tbr1; red - Fezf2 (assay MGI:7620477); green - Satb2 (assay MGI:7620478).
Structure Level Pattern Image Note
TS28: cortical layer V Present S4A Increased immunostaining was observed in the mutant.
TS28: cortical layer VI Present S4A Increased immunostaining was observed in the mutant.
Specimen S4E: postnatal; Bcl11btm1Jpk/Bcl11btm1Jpk, Fezf2tm1Skm/Fezf2tm1Skm (more )
Structure Level Pattern Image Note
TS28: cortical layer VI Present S4E Normal immunostaining was observed in layer 6.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory