Reporter:	alkaline phosphatase
	Direct Detection

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Present	Regionally restricted	2A	Expression was detected in the pyramidal tract.

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Weak	Regionally restricted	2B	The mutant showed a dramatic decrease in reporter staining in the pyramidal tract.

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Present	Regionally restricted	2C	Expression was detected in the pyramidal tract.

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Absent		2D

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Present	Regionally restricted	2E	Expression was detected in the pyramidal tract and pyramidal decussation.

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Weak	Regionally restricted	2F	There was a striking reduction in the number of reporter positive axons in the pyramidal tract.

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Present	Regionally restricted	2G	Expression was detected in the pyramidal tract and pyramidal decussation.

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Absent		2H	No expression was detected in the pyramidal tract.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Present		2I

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Present		2J

Structure	Level	Pattern	Image	Note
TS28: nerve fiber tract	Present	Regionally restricted	2K	Callosal axons in double mutants fail to project across the corpus callosum at P0.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong		6A

Structure	Level	Pattern	Image	Note
TS28: thalamus	Strong		6B

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Weak		6C	Satb2 mutants show a reduction in PLAP+ axons traversing the striatum.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Trace		6D	Almost no expression was observed in the mutant thalamus.

Structure	Level	Pattern	Image	Note
TS28: anterior commissure	Strong		6E
TS28: corpus striatum	Strong		6E

Structure	Level	Pattern	Image	Note
TS28: thalamus	Strong		6F

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Weak		6G	Expression was reduced in the mutant.
TS28: anterior commissure	Strong		6G	Expression remained robust in the mutant.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Weak		6H	Expression was reduced in the mutant.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong		6I

Structure	Level	Pattern	Image	Note
TS28: thalamus	Strong		6J

Structure	Level	Pattern	Image	Note
TS26: corpus striatum	Present		S4F
TS26: nerve fiber tract	Present	Regionally restricted	S4F	Only a narrow band of PLAP+ axons extend past the striatum, and no PLAP+ axons were detected at the level of the pyramidal tract at E18.

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Present	Regionally restricted	S4G	PLAP+ axons extend along the pyramidal tract and reach the pyramidal decussation.
TS28: pyramidal decussation	Present		S4G

Structure	Level	Pattern	Image	Note
TS28: pyramidal decussation	Present		S4H	PLAP+ axons reach the pyramidal decussation.
TS28: dorsal corticospinal tract	Absent		S4H	PLAP+ axons fail to extend past the decussation into the spinal cord.

Structure	Level	Pattern	Image	Note
TS27: cerebral peduncle	Present		S4I
TS27: corpus striatum	Present		S4I
TS27: thalamus	Present		S4I

Structure	Level	Pattern	Image	Note
TS27: corticospinal tract	Present	Regionally restricted	S4J	PLAP+ axons project normally into the pyramidal tract.

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Present		S4K	PLAP-stained axons appeared disorganized in the striatum.
TS27: nerve fiber tract	Present	Regionally restricted	S4K , S4L	PLAP-stained axons appeared disorganized in the striatum, and no PLAP+ axons were detected at the level of the pons.