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Gene Expression Data
Assay Details
Assay
Reference: J:192361 Srinivasan K, et al., A network of genetic repression and derepression specifies projection fates in the developing neocortex. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19071-8
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7620251
Gene symbol: Fezf2
Gene name: Fez family zinc finger 2
Reporter: alkaline phosphatase
Direct Detection
Results
Specimen 2A: postnatal day 4; Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm2Rug (more )
Structure Level Pattern Image Note
TS28: corticospinal tract Present Regionally restricted 2A Expression was detected in the pyramidal tract.
Specimen 2B: postnatal day 4; Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm2Rug (more )
Structure Level Pattern Image Note
TS28: corticospinal tract Weak Regionally restricted 2B The mutant showed a dramatic decrease in reporter staining in the pyramidal tract.
Specimen 2C: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: corticospinal tract Present Regionally restricted 2C Expression was detected in the pyramidal tract.
Specimen 2D: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: corticospinal tract Absent 2D
Specimen 2E: postnatal day 4; Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm2Rug (more )
Structure Level Pattern Image Note
TS28: corticospinal tract Present Regionally restricted 2E Expression was detected in the pyramidal tract and pyramidal decussation.
Specimen 2F: postnatal day 4; Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm2Rug (more )
Structure Level Pattern Image Note
TS28: corticospinal tract Weak Regionally restricted 2F There was a striking reduction in the number of reporter positive axons in the pyramidal tract.
Specimen 2G: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: corticospinal tract Present Regionally restricted 2G Expression was detected in the pyramidal tract and pyramidal decussation.
Specimen 2H: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: corticospinal tract Absent 2H No expression was detected in the pyramidal tract.
Specimen 2I: postnatal day 4; Fezf2tm1Skm/Fezf2tm1Skm (more )
Structure Level Pattern Image Note
TS28: corpus callosum Present 2I
Specimen 2J: postnatal day 4; Fezf2tm1Skm/Fezf2tm1Skm (more )
Structure Level Pattern Image Note
TS28: corpus callosum Present 2J
Specimen 2K: postnatal day 4; Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm1Rug (more )
Note: Double labeled: red - Fezf2 reporter; green - Satb2 reporter (assay MGI:7620184).
Structure Level Pattern Image Note
TS28: nerve fiber tract Present Regionally restricted 2K Callosal axons in double mutants fail to project across the corpus callosum at P0.
Specimen 6A: postnatal; Fezf2tm1Skm/Fezf2+ (more )
Note: Anterior Region
Structure Level Pattern Image Note
TS28: corpus striatum Strong 6A
Specimen 6B: postnatal; Fezf2tm1Skm/Fezf2+ (more )
Note: Posterior Region
Structure Level Pattern Image Note
TS28: thalamus Strong 6B
Specimen 6C: postnatal; Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm1Rug (more )
Note: Anterior Region
Structure Level Pattern Image Note
TS28: corpus striatum Weak 6C Satb2 mutants show a reduction in PLAP+ axons traversing the striatum.
Specimen 6D: postnatal; Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm1Rug (more )
Note: Posterior Region
Structure Level Pattern Image Note
TS28: thalamus Trace 6D Almost no expression was observed in the mutant thalamus.
Specimen 6E: postnatal; Fezf2tm1Skm/Fezf2tm1Skm (more )
Note: Anterior Region
Structure Level Pattern Image Note
TS28: anterior commissure Strong 6E
TS28: corpus striatum Strong 6E
Specimen 6F: postnatal; Fezf2tm1Skm/Fezf2tm1Skm (more )
Note: Posterior Region
Structure Level Pattern Image Note
TS28: thalamus Strong 6F
Specimen 6G: postnatal; Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm1Rug (more )
Note: Anterior Region
Structure Level Pattern Image Note
TS28: corpus striatum Weak 6G Expression was reduced in the mutant.
TS28: anterior commissure Strong 6G Expression remained robust in the mutant.
Specimen 6H: postnatal; Fezf2tm1Skm/Fezf2tm1Skm, Satb2tm1Rug/Satb2tm1Rug (more )
Note: Posterior Region
Structure Level Pattern Image Note
TS28: thalamus Weak 6H Expression was reduced in the mutant.
Specimen 6I: postnatal; Bcl11btm1Jpk/Bcl11btm1Jpk, Fezf2tm1Skm/Fezf2+ (more )
Note: Anterior Region
Structure Level Pattern Image Note
TS28: corpus striatum Strong 6I
Specimen 6J: postnatal; Bcl11btm1Jpk/Bcl11btm1Jpk, Fezf2tm1Skm/Fezf2+ (more )
Note: Posterior Region
Structure Level Pattern Image Note
TS28: thalamus Strong 6J
Specimen S4F: embryonic day 18.0; Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm1Rug (more )
Structure Level Pattern Image Note
TS26: corpus striatum Present S4F
TS26: nerve fiber tract Present Regionally restricted S4F Only a narrow band of PLAP+ axons extend past the striatum, and no PLAP+ axons were detected at the level of the pyramidal tract at E18.
Specimen S4G: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: corticospinal tract Present Regionally restricted S4G PLAP+ axons extend along the pyramidal tract and reach the pyramidal decussation.
TS28: pyramidal decussation Present S4G
Specimen S4H: postnatal day 4; Emx1tm1(cre)Krj/Emx1+, Fezf2tm1Skm/Fezf2+, Satb2tm1Rug/Satb2tm2Rug (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: pyramidal decussation Present S4H PLAP+ axons reach the pyramidal decussation.
TS28: dorsal corticospinal tract Absent S4H PLAP+ axons fail to extend past the decussation into the spinal cord.
Specimen S4I: postnatal day 0; Bcl11btm1Jpk/Bcl11b+, Fezf2tm1Skm/Fezf2+ (more )
Structure Level Pattern Image Note
TS27: cerebral peduncle Present S4I
TS27: corpus striatum Present S4I
TS27: thalamus Present S4I
Specimen S4J: postnatal day 0; Bcl11btm1Jpk/Bcl11b+, Fezf2tm1Skm/Fezf2+ (more )
Structure Level Pattern Image Note
TS27: corticospinal tract Present Regionally restricted S4J PLAP+ axons project normally into the pyramidal tract.
Specimen S4K: postnatal day 0; Bcl11btm1Jpk/Bcl11btm1Jpk, Fezf2tm1Skm/Fezf2+ (more )
Structure Level Pattern Image Note
TS27: corpus striatum Present S4K PLAP-stained axons appeared disorganized in the striatum.
TS27: nerve fiber tract Present Regionally restricted S4K , S4L PLAP-stained axons appeared disorganized in the striatum, and no PLAP+ axons were detected at the level of the pons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory