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Gene Expression Data
Assay Details
Assay
Reference: J:277128 Shiromoto Y, et al., GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. Biol Reprod. 2019 Jul 1;101(1):248-256
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7619098
Gene symbol: Piwil2
Gene name: piwi-like RNA-mediated gene silencing 2
Antibody: Anti-Mili (2071)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen S2A Gpat2+/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2+ (more )
Note: Double labeled: red - Piwil2; green - Gpat2 (assay MGI:7619100).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2A merge Gpat2+/delta , S2A Piwil2 Gpat2+/delta Expression was detected in a granular pattern in prospermatogonia. Gpat2 staining was adjacent or co-localized with Piwil2 immunostaining.
Specimen S2A Gpat2delta/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2tm1.1Nkn (more )
Note: Double labeled: red - Piwil2; green - Gpat2 (assay MGI:7619100).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2A Piwil2 Gpat2delta/delta , S2A merge Gpat2delta/delta The granular staining pattern was retained in the prospermatogonia of the Gpat2 mutant.
Specimen S2A Piwil2-/-: embryonic day 16.5; Piwil2tm1Nkn/Piwil2tm1Nkn (more )
Note: Double labeled: red - Piwil2; green - Gpat2 (assay MGI:7619100).
Structure Level Pattern Image Note
TS24: germ cell of testis Absent S2A merge Piwil2-/- , S2A Piwil2 Piwil2-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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