About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:277128 Shiromoto Y, et al., GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. Biol Reprod. 2019 Jul 1;101(1):248-256
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7619098
Gene symbol: Piwil2
Gene name: piwi-like RNA-mediated gene silencing 2
Antibody: Anti-Mili (2071)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen S2A Gpat2+/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2+ (more )
Note: Double labeled: red - Piwil2; green - Gpat2 (assay MGI:7619100).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2A merge Gpat2+/delta , S2A Piwil2 Gpat2+/delta Expression was detected in a granular pattern in prospermatogonia. Gpat2 staining was adjacent or co-localized with Piwil2 immunostaining.
Specimen S2A Gpat2delta/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2tm1.1Nkn (more )
Note: Double labeled: red - Piwil2; green - Gpat2 (assay MGI:7619100).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2A Piwil2 Gpat2delta/delta , S2A merge Gpat2delta/delta The granular staining pattern was retained in the prospermatogonia of the Gpat2 mutant.
Specimen S2A Piwil2-/-: embryonic day 16.5; Piwil2tm1Nkn/Piwil2tm1Nkn (more )
Note: Double labeled: red - Piwil2; green - Gpat2 (assay MGI:7619100).
Structure Level Pattern Image Note
TS24: germ cell of testis Absent S2A merge Piwil2-/- , S2A Piwil2 Piwil2-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory