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Gene Expression Data
Assay Details
Assay
Reference: J:277128 Shiromoto Y, et al., GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. Biol Reprod. 2019 Jul 1;101(1):248-256
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7619100
Gene symbol: Gpat2
Gene name: glycerol-3-phosphate acyltransferase 2, mitochondrial
Antibody: Anti-Gpat2 (4H11)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S2A Gpat2+/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2+ (more )
Note: Double labeled: green - Gpat2; red - Piwil2 (assay MGI:7619098).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2A merge Gpat2+/delta , S2A Gpat2 Gpat2+/delta Expression was detected in a granular pattern in prospermatogonia. Gpat2 staining was adjacent or co-localized with Piwil2 immunostaining.
Specimen S2A Gpat2delta/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2tm1.1Nkn (more )
Note: Double labeled: green - Gpat2; red - Piwil2 (assay MGI:7619098).
Structure Level Pattern Image Note
TS24: germ cell of testis Absent S2A merge Gpat2delta/delta , S2A Gpat2 Gpat2delta/delta
Specimen S2A Piwil2-/-: embryonic day 16.5; Piwil2tm1Nkn/Piwil2tm1Nkn (more )
Note: Double labeled: green - Gpat2; red - Piwil2 (assay MGI:7619098).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2A Gpat2 Piwil2-/- , S2A merge Piwil2-/- The granular staining pattern was retained in the prospermatogonia of the Piwil2 mutant.
Specimen S2B Gpat2+/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2+ (more )
Note: Double labeled: green - Gpat2; red - Tdrd1 (assay MGI:7619099).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2B merge Gpat2+/delta , S2B Gpat2 Gpat2+/delta Expression was detected in a granular pattern in prospermatogonia. Gpat2 staining was adjacent or co-localized with Tdrd1 immunostaining.
Specimen S2B Gpat2delta/delta: embryonic day 16.5; Gpat2tm1.1Nkn/Gpat2tm1.1Nkn (more )
Note: Double labeled: green - Gpat2; red - Tdrd1 (assay MGI:7619099).
Structure Level Pattern Image Note
TS24: germ cell of testis Absent S2B Gpat2 Gpat2delta/delta , S2B merge Gpat2delta/delta
Specimen S2B Piwil2-/-: embryonic day 16.5; Piwil2tm1Nkn/Piwil2tm1Nkn (more )
Note: Double labeled: green - Gpat2; red - Tdrd1 (assay MGI:7619099).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Regionally restricted S2B merge Piwil2-/- , S2B Gpat2 Piwil2-/- The granular staining pattern was retained in the prospermatogonia of the Piwil2 mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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