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Gene Expression Data
Assay Details
Assay
Reference: J:331053 Chandrasekaran P, et al., CXCL12 defines lung endothelial heterogeneity and promotes distal vascular growth. Development. 2022 Nov 1;149(21):dev200909
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7616684
Gene symbol: Cxcl12
Gene name: C-X-C motif chemokine ligand 12
Reporter: RFP
Antibody: anti-RFP (600-401-379)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1A: embryonic day 12.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS20: pulmonary artery Strong Regionally restricted 1A High levels of expression in pulmonary arteries marking two primary intrapulmonary branches.
Specimen 1B: embryonic day 13.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS21: pulmonary artery Present Regionally restricted 1B Expression in secondary branching from primary intrapulmonary arteries, and in small, thin tubular sprouts growing toward lung periphery.
Specimen 1C: embryonic day 13.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS21: lung vascular element Present Regionally restricted 1C Expression in secondary branching from primary intrapulmonary arteries, and in small, thin tubular sprouts growing toward lung periphery.
Specimen 1D: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted 1D Expression in increased secondary branching, along with tertiary and quaternary arterial branches.
Specimen 1E: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 1E Expression in arterial tree undergoing significant growth and futher branching complexity.
Specimen S1A: embryonic day 12.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Vwf (assay MGI:7616685).
Structure Level Pattern Image Note
TS20: lung vascular element Present Regionally restricted S1A HIghly expressed throughout arterial endothelium, with additional expression in mesenchyme.
Specimen S1B: embryonic day 13.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Vwf (assay MGI:7616685).
Structure Level Pattern Image Note
TS21: lung vascular element Strong Regionally restricted S1B HIghly expressed throughout arterial endothelium, with additional expression in mesenchyme.
Specimen S1C: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
Structure Cell Type Level Pattern Image Note
TS23: pulmonary artery blood vessel smooth muscle cell Present S1C
TS23: pulmonary artery lung endothelial cell Present S1C
Specimen S1D: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted S1D No expression in venous endothelium.
Specimen S1E: embryonic day 17.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
Structure Cell Type Level Pattern Image Note
TS25: pulmonary artery blood vessel smooth muscle cell Present S1E
TS25: pulmonary artery lung endothelial cell Present S1E
Specimen S1F: embryonic day 17.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686); green - Erg (assay MGI:7616687).
Structure Level Pattern Image Note
TS25: lung vascular element Present Regionally restricted S1F No expression in venous endothelium.
Specimen S1G: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
Structure Cell Type Level Pattern Image Note
TS26: pulmonary artery blood vessel smooth muscle cell Present S1G
TS26: pulmonary artery lung endothelial cell Present S1G
Specimen S1H: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S1H No expression in venous endothelium.
Specimen S1I: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Double labeled: red - Cxcl12 reporter; white - Emcn (assay MGI:7616686).
Structure Level Pattern Image Note
TS26: pulmonary artery Present S1I
Specimen S1J: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; green - Acta2 (assay MGI:7616688); white - Emcn (assay MGI:7616686).
Structure Level Pattern Image Note
TS26: lung vascular element Ambiguous S1J Expression not described.
Specimen 3F: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: pulmonary artery Present Regionally restricted 3F Expression in proximal artery branch.
TS26: lung vascular element Present Regionally restricted 3F Expression in arterial tree from distal tip of secondary branch to quinary branches.
Specimen 3G: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 3G Expression in arterial endothelium.
Specimen 3H,K: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 3K , 3H Expression in proximal region of arterial tree.
Specimen 3I,N: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Triple labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703); white - Gkn3 (assay MGI:7616702).
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 3N , 3I Expression in distal region of arterial tree.
Specimen S3A: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Double labeled: red - Cxcl12 reporter; white - Gkn3 (assay MGI:7616702).
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted S3A
Specimen S3C: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Note: Double labeled: red - Cxcl12 reporter; green - Alox12 (assay MGI:7616703).
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted S3C
Specimen S3F: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted S3F Expression in proximal region of arterial tree.
Specimen S3I: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted S3I Expression in middle region of arterial tree.
Specimen S3L: embryonic day 15.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS23: lung vascular element Present Regionally restricted S3L Expression in distal region of arterial tree.
Specimen 5A: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present 5A p
Specimen 5B: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 5B Proximal and distal branching defects.
Specimen 5D: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present 5D
Specimen 5F: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 5F Increase in total branching number.
Specimen 5J (upper): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present 5J (upper)
Specimen 5J (lower): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 5J (lower) Authors report changes in vessel density.
Specimen S5A: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present S5A
Specimen S5B: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S5B Distally, arteries lack directionality toward periphery.
Specimen S5F (upper): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S5F (upper) Expression in capillaries.
Specimen S5F (lower): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S5F (lower) Capillary density significantly reduced compared to control.
Specimen S5G (upper): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S5G (upper) Expression in veins.
Specimen S5G (lower): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S5G (lower) Density of veins unchange compared to control.
Specimen S5H (upper): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+ (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S5H (upper) Expression in arteries.
Specimen S5H (lower): embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm2.1Sjm (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted S5H (lower) Arterial density modestly reduced compared to control.
Specimen 7A: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12+, Tg(Tek-cre)12Flv/0 (more )
Structure Level Pattern Image Note
TS26: lung vascular element Present 7A
Specimen 7B: embryonic day 18.5; Cxcl12tm2.1Sjm/Cxcl12tm1.1Sjm, Tg(Tek-cre)12Flv/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: lung vascular element Present Regionally restricted 7B Authors report primary branching defects, and decreased secondary branch numbers.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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