About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:213418 Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
Assay type: RNA in situ
MGI Accession ID: MGI:7615140
Gene symbol: Alx4
Gene name: aristaless-like homeobox 4
Probe: Alx4 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2Y: embryonic day 10.5-10.75 (more )
Note: 36-39-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 2Y Expression marked the anterior regions.
Specimen 2Z: embryonic day 10.5-10.75; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: 36-39-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 2Z Greatly reduced expression compared with the control.
Specimen S3E: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted S3E Expressed in the anterior region.
Specimen S3F: embryonic day 10.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted S3F Reduced expression in the anterior region compared with the control.
Specimen S3G: embryonic day 10.5; Kif7tm1.2Hui/Kif7tm1.2Hui (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted S3G Reduced expression in the anterior region compared with the control.
Specimen S3H: embryonic day 10.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui, Kif7tm1.2Hui/Kif7tm1.2Hui (more )
Structure Level Pattern Image Note
TS17: forelimb bud Weak Regionally restricted S3H Almost lost expression in the anterior region compared with the control.
Specimen S3I: embryonic day 9.25 (more )
Structure Level Pattern Image Note
TS15: forelimb bud Present Regionally restricted S3I-D , S3I-L Expressed in the anterior region.
Specimen S3J: embryonic day 9.25; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Structure Level Pattern Image Note
TS15: forelimb bud Weak Regionally restricted S3J-D , S3J-L Reduced expression in the anterior region compared with the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
01/28/2026
MGI 6.24 		The Jackson Laboratory