Gene Expression Data
Assay Details
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Assay
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Reference:
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J:213418
Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7615135
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2M:
embryonic day 10.5-10.75
(more  )
Note: 36-39-somite stage
Specimen
2M:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5-10.75
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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36-39-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2M
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Expressed by a group of cells in the posterior aspect of limb bud mesenchyme called the zone of polarizing activity (ZPA).
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Specimen
2N:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Irx3tm3.1Hui/Irx3tm3.1Hui
Irx5tm3Hui/Irx5tm3Hui
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Age:
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embryonic day 10.5-10.75
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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36-39-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2N
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Signaling center was anteriorly shifted, compared with the control.
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Specimen
4A:
embryonic day 10.0
(more )
Note: 30-somite stage
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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30-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
4A
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
4B:
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
4B
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
4C:
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
4C
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
4D:
embryonic day 10.5
(more )
Note: 38-somite stage
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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38-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
4D
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4A:
embryonic day 9.75
(more )
Note: 28-somite stage
Specimen
S4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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28-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4A
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4B:
embryonic day 10.0
(more )
Note: 30-somite stage
Specimen
S4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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30-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4B
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4C:
embryonic day 10.0
(more )
Note: 32-somite stage
Specimen
S4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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32-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4C
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4D:
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
S4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Present |
Regionally restricted |
S4D
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4E:
embryonic day 10.5
(more )
Note: 36-somite stage
Specimen
S4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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36-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
S4E
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4F:
embryonic day 10.0
(more )
Note: 32-somite stage
Specimen
S4F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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32-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
S4F
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4G:
embryonic day 10.0
(more )
Note: 34-somite stage
Specimen
S4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
S4G
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4H:
embryonic day 10.5
(more )
Note: 36-somite stage
Specimen
S4H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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36-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4H
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4I:
embryonic day 10.5
(more )
Note: 38-somite stage
Specimen
S4I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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38-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
S4I
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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Specimen
S4J:
embryonic day 11.0
(more )
Note: 40-somite stage
Specimen
S4J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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40-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud |
Present |
Regionally restricted |
S4J
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Expressed more posteriorly restricted in the forelimb relative to the hindlimb.
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