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Gene Expression Data
Assay Details
Assay
Reference: J:213418 Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
Assay type: RNA in situ
MGI Accession ID: MGI:7615135
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2M: embryonic day 10.5-10.75 (more )
Note: 36-39-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud mesenchyme Present Regionally restricted 2M Expressed by a group of cells in the posterior aspect of limb bud mesenchyme called the zone of polarizing activity (ZPA).

Specimen 2N: embryonic day 10.5-10.75; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: 36-39-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud mesenchyme Present Regionally restricted 2N Signaling center was anteriorly shifted, compared with the control.

Specimen 4A: embryonic day 10.0 (more )
Note: 30-somite stage
Structure Level Pattern Image Note
TS16: forelimb bud Present Regionally restricted 4A Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen 4B: embryonic day 10.0 (more )
Note: 34-somite stage
Structure Level Pattern Image Note
TS16: forelimb bud Present Regionally restricted 4B Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen 4C: embryonic day 10.0 (more )
Note: 34-somite stage
Structure Level Pattern Image Note
TS16: hindlimb bud Present Regionally restricted 4C Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen 4D: embryonic day 10.5 (more )
Note: 38-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 4D Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4A: embryonic day 9.75 (more )
Note: 28-somite stage
Structure Level Pattern Image Note
TS16: forelimb bud Present Regionally restricted S4A Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4B: embryonic day 10.0 (more )
Note: 30-somite stage
Structure Level Pattern Image Note
TS16: forelimb bud Present Regionally restricted S4B Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4C: embryonic day 10.0 (more )
Note: 32-somite stage
Structure Level Pattern Image Note
TS16: forelimb bud Present Regionally restricted S4C Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4D: embryonic day 10.0 (more )
Note: 34-somite stage
Structure Level Pattern Image Note
TS16: forelimb bud Present Regionally restricted S4D Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4E: embryonic day 10.5 (more )
Note: 36-somite stage
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted S4E Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4F: embryonic day 10.0 (more )
Note: 32-somite stage
Structure Level Pattern Image Note
TS16: hindlimb bud Present Regionally restricted S4F Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4G: embryonic day 10.0 (more )
Note: 34-somite stage
Structure Level Pattern Image Note
TS16: hindlimb bud Present Regionally restricted S4G Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4H: embryonic day 10.5 (more )
Note: 36-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4H Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4I: embryonic day 10.5 (more )
Note: 38-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted S4I Expressed more posteriorly restricted in the forelimb relative to the hindlimb.

Specimen S4J: embryonic day 11.0 (more )
Note: 40-somite stage
Structure Level Pattern Image Note
TS18: hindlimb bud Present Regionally restricted S4J Expressed more posteriorly restricted in the forelimb relative to the hindlimb.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory