About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:213418 Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
Assay type: RNA in situ
MGI Accession ID: MGI:7615132
Gene symbol: Gli3
Gene name: GLI-Kruppel family member GLI3
Probe: Gli3 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2E: embryonic day 9.75 (more )
Note: 29-somite stage
Structure Level Pattern Image Note
TS16: hindlimb bud Present Regionally restricted 2E Expression marked the anterior regions.
Specimen 2F: embryonic day 9.75; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: 29-somite stage
Structure Level Pattern Image Note
TS16: hindlimb bud Present Regionally restricted 2F The posterior extent of expression was diminished in the mutant.
Specimen 2W: embryonic day 10.5-10.75 (more )
Note: 36-39-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 2W Expression marked the anterior regions.
Specimen 2X: embryonic day 10.5-10.75; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: 36-39-somite stage
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 2X Greatly reduced expression compared with the control.
Specimen 4N: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 4N Expressed in the anterior region.
Specimen 4O: embryonic day 10.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 4O Reduced expression compared with the control.
Specimen 4P: embryonic day 10.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui, Shhtm1Chg/Shh+ (more )
Structure Level Pattern Image Note
TS17: hindlimb bud Present Regionally restricted 4P Expression domain was expanded compared with the DKO mutants.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory