Gene Expression Data
Assay Details
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Assay
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Reference:
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J:213418
Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7615127
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Gene symbol:
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Irx5
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Gene name:
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Iroquois homeobox 5
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Reporter:
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GFP
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Direct Detection
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Results
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Specimen
1D:
embryonic day 9.5; Irx5tm3Hui/Irx5 +
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Irx5tm3Hui/Irx5+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
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1D
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Expression domains overlapped with that of Irx3.
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Specimen
1H:
embryonic day 10.0; Irx5tm3Hui/Irx5 +
(more )
Specimen
1H:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Irx5tm3Hui/Irx5+
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
1H
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Expressed in the anterior region.
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Specimen
1I:
embryonic day 10.5; Irx5tm3Hui/Irx5 +
(more )
Specimen
1I:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Irx5tm3Hui/Irx5+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
1I
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Expressed in the anterior region.
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Specimen
1J:
embryonic day 12.5; Irx5tm3Hui/Irx5 +
(more )
Specimen
1J:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Irx5tm3Hui/Irx5+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
1J
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Expressed in the proximal-anterior limb.
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Specimen
not shown:
embryonic day 8.5; Irx5tm3Hui/Irx5 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Irx5tm3Hui/Irx5+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools