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Gene Expression Data
Assay Details
Assay
Reference: J:293098 Jafree DJ, et al., Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease. Elife. 2019 Dec 6;8:e48183
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7614751
Gene symbol: Pdpn
Gene name: podoplanin
Antibody: Anti-Podoplanin clone D2-40 (M361929-2)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Depending on the different antibody combination in the double/triple labeling, another antibody (MGI:5476703) could be used.
Results
Specimen 2B PROX1/Podoplanin: embryonic day 16.5 (more )
Note: Double labeled: green - Pdpn; purple - Prox1 (assay MGI:7614746).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 2B Podoplanin , 2B merge middle Coexpressed with Prox1 in cell clusters.
TS24: maturing glomerular tuft Present 2B Podoplanin , 2B merge middle

Specimen 1-S1 PROX1/Podoplanin: embryonic day 16.5 (more )
Note: Double labeled: green - Pdpn; purple - Prox1 (assay MGI:7614746).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Strong 1-S1_merge middle left , 1-S1_merge middle right , 1-S1_Podoplanin Coexpressed with Prox1 in the lymphatic plexus.
TS24: metanephros Present 1-S1_merge middle left

Specimen 1-S2A: embryonic day 16.5 (more )
Note: Triple labeled: green - Pdpn; purple - Prox1 (assay MGI:7614746); blue - Upk3a (assay MGI:7614779).
Structure Cell Type Level Pattern Image Note
TS24: renal lymphatic endothelial cell of lymphatic vessel Present 1-S2A merge left , 1-S2A merge right , 1-S2A Podoplanin The positive ring-like lymphatic anastomosis resided under the nascent Upk3a+ renal pelvis.
TS24: metanephros Present 1-S2A Podoplanin , 1-S2A merge left , 1-S2A merge right

Specimen 1-S2D: embryonic day 18.5 (more )
Note: Triple labeled: green - Pdpn; blue - Aqp2 (assay MGI:7614774); purple - Prox1 (assay MGI:7614746).
Structure Cell Type Level Pattern Image Note
TS26: metanephros Present 1-S2D merge right , 1-S2D Podoplanin , 1-S2D merge left
TS26: renal lymphatic endothelial cell of lymphatic vessel Present 1-S2D merge left , 1-S2D Podoplanin , 1-S2D merge right Coexpressed with Prox1 in the lymphatic vessels. No lymphatic vessels in the renal medulla region (Aqp2+), nor near or within the renal capsule.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory