Gene Expression Data
Assay Details
Assay
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Reference:
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J:320376
Kleijer KTE, et al., Structural abnormalities in the primary somatosensory cortex and a normal behavioral profile in Contactin-5 deficient mice. Cell Adh Migr. 2018 Jan 2;12(1):5-18
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7611789
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Gene symbol:
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Cntn5
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Gene name:
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contactin 5
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Reporter:
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lacZ
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Probe:
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lacZ probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3E:
(close )
Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Cntn5tm1Kwat/Cntn5tm1Kwat
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
Regionally restricted |
3E
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Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.
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TS28: primary somatosensory cortex |
Present |
Regionally restricted |
3E
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Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.
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Specimen
3E':
(close )
Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Cntn5tm1Kwat/Cntn5tm1Kwat
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
Regionally restricted |
3E'
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Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.
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TS28: primary somatosensory cortex |
Present |
Regionally restricted |
3E'
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Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.
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Specimen
3G:
postnatal day 7; Cntn5tm1Kwat/ Cntn5tm1Kwat
(more )
Note: This specimen was described as double labeled in the text, but the colors for beta-galactosidase and parvalbumin were not specified.
Specimen
3G:
(close )
Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Cntn5tm1Kwat/Cntn5tm1Kwat
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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This specimen was described as double labeled in the text, but the colors for beta-galactosidase and parvalbumin were not specified.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
cortical interneuron |
Present |
Regionally restricted |
3G
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Both parvalbumin-positive interneurons and parvalbumin-negative cells were found among the Cntn5-ablated cells in layer V.
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TS28: cortical layer V |
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Present |
Regionally restricted |
3G
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Both parvalbumin-positive interneurons and parvalbumin-negative cells were found among the Cntn5-ablated cells in layer V.
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