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Gene Expression Data
Assay Details
Assay
Reference: J:320376 Kleijer KTE, et al., Structural abnormalities in the primary somatosensory cortex and a normal behavioral profile in Contactin-5 deficient mice. Cell Adh Migr. 2018 Jan 2;12(1):5-18
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7611789
Gene symbol: Cntn5
Gene name: contactin 5
Reporter: lacZ
Probe: lacZ probe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3E: postnatal adult; Cntn5tm1Kwat/Cntn5tm1Kwat (more )
Structure Level Pattern Image Note
TS28: cortical layer V Present Regionally restricted 3E Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.
TS28: primary somatosensory cortex Present Regionally restricted 3E Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.

Specimen 3E': postnatal adult; Cntn5tm1Kwat/Cntn5tm1Kwat (more )
Structure Level Pattern Image Note
TS28: cortical layer V Present Regionally restricted 3E' Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.
TS28: primary somatosensory cortex Present Regionally restricted 3E' Comparison with the in situ hybridization in wild type animals (staining layer IV), the beta-galactosidase-stained Cntn5-ablated cells were misplaced. The ectopic Cntn5-/- cells were found in the lower layers of the primary somatosensory cortex.

Specimen 3G: postnatal day 7; Cntn5tm1Kwat/Cntn5tm1Kwat (more )
Note: This specimen was described as double labeled in the text, but the colors for beta-galactosidase and parvalbumin were not specified.
Structure Cell Type Level Pattern Image Note
TS28: cortical layer V cortical interneuron Present Regionally restricted 3G Both parvalbumin-positive interneurons and parvalbumin-negative cells were found among the Cntn5-ablated cells in layer V.
TS28: cortical layer V Present Regionally restricted 3G Both parvalbumin-positive interneurons and parvalbumin-negative cells were found among the Cntn5-ablated cells in layer V.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory