Gene Expression Data
Assay Details
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Assay
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Reference:
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J:145300
Kim AY, et al., Pirt, a phosphoinositide-binding protein, functions as a regulatory subunit of TRPV1. Cell. 2008 May 2;133(3):475-85
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7611628
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Gene symbol:
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Pirt
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Gene name:
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phosphoinositide-interacting regulator of transient receptor potential channels
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Reporter:
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GFP
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Antibody:
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anti-GFP (#A11122)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
S3A:
postnatal adult; Pirttm1Xzd/Pirt +
(more  )
Note: L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - Calca (assay MGI:7611621).
Specimen
S3A:
(close  )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - Calca (assay MGI:7611621).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: dorsal root ganglion |
sensory neuron of dorsal root ganglion |
Present |
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S3A
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GFP-immunoreactive cells were detected. Co-labeling with the sensory neuron marker, Calca was observed.
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Specimen
S3B:
postnatal adult; Pirttm1Xzd/Pirt +
(more )
Note: L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - IB4.
Specimen
S3B:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - IB4.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: dorsal root ganglion |
sensory neuron of dorsal root ganglion |
Present |
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S3B
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GFP-immunoreactive cells were detected. Co-labeling with isolectin B4 was observed.
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Specimen
S3C:
postnatal adult; Pirttm1Xzd/Pirt +
(more )
Note: L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - Nefh (assay MGI:7611627).
Specimen
S3C:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - Nefh (assay MGI:7611627).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: dorsal root ganglion |
sensory neuron of dorsal root ganglion |
Present |
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S3C
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GFP-immunoreactive cells were detected. Co-labeling with the sensory neuron marker, Nefh was observed.
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Specimen
S3D:
postnatal adult; Pirttm1Xzd/Pirt +
(more )
Note: L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - Trpv1 (assay MGI:7610923).
Specimen
S3D:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
L4-L6 lumbar region dorsal root ganglion. Double labeled: green - Pirt reporter; red - Trpv1 (assay MGI:7610923).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: dorsal root ganglion |
sensory neuron of dorsal root ganglion |
Present |
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S3D
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GFP-immunoreactive cells were detected. Co-labeling with the sensory neuron marker, Trpv1 was observed.
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Specimen
S3E:
postnatal adult; Pirttm1Xzd/Pirt +
(more )
Note: L4-L6 lumbar region spinal cord. Double labeled: green - Pirt reporter; red - Calca (assay MGI:7611621).
Specimen
S3E:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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L4-L6 lumbar region spinal cord. Double labeled: green - Pirt reporter; red - Calca (assay MGI:7611621).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord lamina |
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Present |
Regionally restricted |
S3E lower center
,
S3E lower right
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S3E
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Labeled axons of Pirt-expressing neurons were observed projecting to lamina I & II of the dorsal spinal cord. Co-expression with Calca was observed in lamina I.
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| TS28: spinal cord |
neuron |
Present |
Regionally restricted |
S3E
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S3E lower center
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S3E lower right
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Expression was detected in dorsal spinal cord.
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Specimen
S3F:
postnatal adult; Pirttm1Xzd/Pirt +
(more )
Note: L4-L6 lumbar region spinal cord. Double labeled: green - Pirt reporter; red - IB4.
Specimen
S3F:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
L4-L6 lumbar region spinal cord. Double labeled: green - Pirt reporter; red - IB4.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord lamina |
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Present |
Regionally restricted |
S3F lower center
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S3F
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S3F lower right
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Labeled axons of Pirt-expressing neurons were observed projecting to lamina I & II of the dorsal spinal cord. Co-expression with isolectin B4 was observed in lamina II.
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| TS28: spinal cord |
neuron |
Present |
Regionally restricted |
S3F
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S3F lower center
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S3F lower right
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Expression was detected in dorsal spinal cord.
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