Gene Expression Data
Assay Details
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Assay
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Reference:
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J:145300
Kim AY, et al., Pirt, a phosphoinositide-binding protein, functions as a regulatory subunit of TRPV1. Cell. 2008 May 2;133(3):475-85
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7611621
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Gene symbol:
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Calca
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Gene name:
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calcitonin/calcitonin-related polypeptide, alpha
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Antibody:
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anti-CGRP (T-4239)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
S2C:
postnatal adult
(more  )
Specimen
S2C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S2D:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirttm1Xzd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S3A:
postnatal adult; Pirttm1Xzd/Pirt +
(more )
Note: L4-L6 lumbar region dorsal root ganglion. Double labeled: red - Calca; green - Pirt reporter (assay MGI:7611628).
Specimen
S3A:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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L4-L6 lumbar region dorsal root ganglion. Double labeled: red - Calca; green - Pirt reporter (assay MGI:7611628).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: dorsal root ganglion |
sensory neuron of dorsal root ganglion |
Present |
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S3A
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Immunoreactivity was detected in sensory neurons. Double labeled cells were observed.
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Specimen
S3E:
postnatal adult; Pirttm1Xzd/Pirt +
(more )
Note: L4-L6 lumbar region spinal cord. Double labeled: red - Calca; green - Pirt reporter (assay MGI:7611628).
Specimen
S3E:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirt+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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L4-L6 lumbar region spinal cord. Double labeled: red - Calca; green - Pirt reporter (assay MGI:7611628).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord lamina |
Present |
Regionally restricted |
S3E
,
S3E lower right
,
S3E lower left
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Expression was detected in lamina I of the dorsal spinal cord. Co-expression was observed.
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Specimen
not shown +/+:
postnatal adult
(more )
Note: Data from Table in 2C.
Specimen
not shown +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Data from Table in 2C.
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Specimen
not shown -/-:
(close )
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Genetic Background:
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B6.129-Pirt1tm1Xzd
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Mutant Allele(s):
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Pirttm1Xzd/Pirttm1Xzd
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Data from Table in 2C.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: dorsal root ganglion |
sensory neuron of dorsal root ganglion |
Present |
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The mutant had normal proportions of immunostained neurons.
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Analysis Tools