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Gene Expression Data
Assay Details
Assay
Reference: J:173957 Tsutsumi M, et al., Characterization of a Novel Mouse Gene Encoding an SYCP3-Like Protein That Relocalizes from the XY Body to the Nucleolus During Prophase of Male Meiosis I. Biol Reprod. 2011 Jul;85(1):165-71
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7610715
Gene symbol: 1700013H16Rik
Gene name: RIKEN cDNA 1700013H16 gene
Antibody: anti-XLR6
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A Spermatocytes Leptotene: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: spermatocyte Present Diffuse 3A sperm Lep XLR6 , 3A sperm Lep merge Diffusely, but not uniformly, distributed throughout the nuclei.
Specimen 3A Spermatocytes Zygotene: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: spermatocyte Present Diffuse 3A sperm Zyg merge , 3A sperm Zyg XLR6 Diffusely, but not uniformly, distributed throughout the nuclei.
Specimen 3A Spermatocytes Pachytene: postnatal adult (more )
Note: Meiotic nuclear spreads. Triple labeled: green - 1700013H16Rik; blue - H2ax (assay MGI:7610717); red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: spermatocyte Strong Regionally restricted 3A sperm Pach merge , 3A sperm Pach XLR6 Expressed in the the XY body (coexpressed with H2ax).
Specimen 3A Spermatocytes Diplotene: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: spermatocyte Strong Regionally restricted 3A sperm Dip XLR6 , 3A sperm Dip merge Expressed in the the XY body.
Specimen 3A Spermatocytes Diakinesis: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: spermatocyte Absent 3A sperm Dia XLR6 , 3A sperm Dia merge Absent in diakinesis stage, accordance with the disappearance of the XY body.
Specimen 3A Oocyte Leptotene: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: oocyte Present Diffuse 3A oocyte Lep XLR6 , 3A oocyte Lep merge Diffusely, but not uniformly, distributed throughout the nuclei.
Specimen 3A Oocyte Zygotene: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: oocyte Present Diffuse 3A oocyte Zyg merge , 3A oocyte Zyg XLR6 Diffusely, but not uniformly, distributed throughout the nuclei.
Specimen 3A Oocyte Pachytene: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: oocyte Absent 3A oocyte Pach merge , 3A oocyte Pach XLR6
Specimen 3A Oocyte Diplotene: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: oocyte Absent 3A oocyte Dip XLR6 , 3A oocyte Dip merge
Specimen 3B Pachytene oocyte: postnatal adult (more )
Note: Meiotic nuclear spreads. Double labeled: green - 1700013H16Rik; red - Sycp3 (assay MGI:7610716).
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 3B XLR6 , 3B merge Signals were observed at the unpaired chromosomes occasionally found in the pachytene oocytes with pairing failure.
Specimen 4A Early-pachy: postnatal adult (more )
Note: Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4A early merge , 4A early XLR6 Expressed in the the XY body (coexpressed with H2ax).
TS28: round spermatid Absent 4A early merge , 4A early XLR6
Specimen 4A Mid-pachy: postnatal adult (more )
Note: Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4A mid XLR6 , 4A mid merge Expressed in the the XY body (coexpressed with H2ax).
TS28: round spermatid Absent 4A mid merge , 4A mid XLR6
TS28: spermatogonium Present 4A mid merge , 4A mid XLR6 Expressed in the nuclei of type B spermatogonia.
Specimen 4A Late-pachy: postnatal adult (more )
Note: Triple labeled: green - 1700013H16Rik; blue - H1f6 (assay MGI:7610719); red - H2ax (assay MGI:7610718).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4A late XLR6 , 4A late merge Concentrated in a distinct nuclear compartment at the center side of the H2ax-stained XY body but could still be observed at the nuclear periphery.
Specimen 4B Late-diplo: postnatal adult (more )
Note: Double labeled: green - 1700013H16Rik; red - H2ax (assay MGI:7610718).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4B merge , 4B XLR6 Signals were separate from those of the XY body and appeared at the center of the nuclei.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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